Identification of a novel two-peptide lantibiotic, Haloduracin, produced by the alkaliphile Bacillus halodurans C-125
Identification of a novel two-peptide lantibiotic, Haloduracin, produced by the alkaliphile Bacillus halodurans C-125
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DOI：
10.1111/j.1574-6968.2006.00539.x
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发表时间：
2007-02-01
影响因子：
2.1
通讯作者：
Ross, R. Paul
中科院分区：
文献类型：
--
作者：
Lawton, Elaine M.;Cotter, Paul D.;Ross, R. Paul
嗜碱细菌嗜碱芽孢杆菌C-125的完整基因组测序显示，存在几个与抗生素多肽产生有关的基因。另外的生物信息学分析确定了总共11个基因，跨越15kbp的区域，潜在地参与了双肽抗生素的生产、修饰、免疫和运输。在确定菌株C-125对多种革兰氏阳性菌具有抗菌活性后，通过多肽纯化、MS和定点突变证明，这种活性确实归因于这些基因编码的抗生素的产生。这种抗菌剂被命名为卤尿酸，这是首次将双肽抗生素的生产与芽孢杆菌联系在一起。这也是第一个由亲碱物种产生的抗生素的例子。
Complete genome sequencing of the alkaliphilic bacterium Bacillus halodurans C-125 revealed the presence of several genes homologous to those involved in the production of lantibiotic peptides. Additional bioinformatic analysis identified a total of eleven genes, spanning a 15 kbp region, potentially involved in the production, modification, immunity and transport of a two-peptide lantibiotic. Having established that strain C-125 exhibited antimicrobial activity against a wide range of Gram-positive bacteria, it was demonstrated through peptide purification, MS and site-directed mutagenesis that this activity was indeed attributable to the production of a lantibiotic encoded by these genes. This antimicrobial has been designated haloduracin and represents the first occasion wherein production of two-peptide lantibiotic has been associated with a Bacillus sp. It is also the first example of a lantibiotic of any kind to be produced by an alkaliphilic species.