Changes in apoptotic microRNA and mRNA expression profiling in Caenorhabditis elegans during the Shenzhou-8 mission.
Changes in apoptotic microRNA and mRNA expression profiling in Caenorhabditis elegans during the Shenzhou-8 mission.
复制标题
神舟八号任务期间秀丽隐杆线虫细胞凋亡 microRNA 和 mRNA 表达谱的变化。
DOI：
10.1093/jrr/rrv050
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发表时间：
2015-11
影响因子：
2
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Gao Y;Li S;Xu D;Wang J;Sun Y
辐射和微重力暴露可诱导microRNA(MiRNA)和mRNA表达异常，但空间条件(包括辐射和微重力)是否激活miRNAs调节细胞凋亡尚不确定。为此，我们研究了ced-1线虫突变体和野生型的miRNome和mRNA的表达，在神舟八号任务期间，这两个突变体都经历了航天、航天1g离心机控制和地面控制。结果表明，在航天和航天控制条件下，未检测到蠕虫的形态变化，但差异表达的miRNA分别从地面控制条件的43条增加到57条和91条。与1g孵育相比，微重力通过减少差异表达的miRNA的数量和意义，改变了miRNA的表达谱。MiRNAs的改变参与了细胞凋亡、神经发生、幼虫发育、ATP代谢和GTPase介导的信号转导的改变。其中，17个可能参与细胞凋亡的改变的miRNAs被筛选出来，并且在不同的空间条件下表现出明显的差异表达特征。通过对miRNA和mRNA的整合分析，miR-797和miR-81可能分别通过靶向Ced-10和DRP-1和HSP-1参与细胞凋亡。与地面条件相比，航天条件通过不同的转录方式调控细胞凋亡，航天条件下有7个核心凋亡基因的表达发生改变，航天控制条件下有8个基因表达发生改变。结果表明，秀丽线虫的miRNA可能参与了对空间环境胁迫的调控，并且在微重力条件下表现出不同于1g空间辐射条件下的行为。
Radiation and microgravity exposure have been proven to induce abnormal apoptosis in microRNA (miRNA) and mRNA expression, but whether space conditions, including radiation and microgravity, activate miRNAs to regulate the apoptosis is undetermined. For that purpose, we investigated miRNome and mRNA expression in the ced-1 Caenorhabditis elegans mutant vs the wild-type, both of which underwent spaceflight, spaceflight 1g-centrifuge control and ground control conditions during the Shenzhou-8 mission. Results showed that no morphological changes in the worms were detected, but differential miRNA expression increased from 43 (ground control condition) to 57 and 91 in spaceflight and spaceflight control conditions, respectively. Microgravity altered miRNA expression profiling by decreasing the number and significance of differentially expressed miRNA compared with 1 g incubation during spaceflight. Alterations in the miRNAs were involved in alterations in apoptosis, neurogenesis larval development, ATP metabolism and GTPase-mediated signal transduction. Among these, 17 altered miRNAs potentially involved in apoptosis were screened and showed obviously different expression signatures between space conditions. By integrated analysis of miRNA and mRNA, miR-797 and miR-81 may be involved in apoptosis by targeting the genes ced-10 and both drp-1 and hsp-1, respectively. Compared with ground condition, space conditions regulated apoptosis though a different manner on transcription, by altering expression of seven core apoptotic genes in spaceflight condition, and eight in spaceflight control condition. Results indicate that, miRNA of Caenorhabditis elegans probably regulates apoptotic gene expression in response to space environmental stress, and shows different behavior under microgravity condition compared with 1 g condition in the presence of space radiation.