Microarray profile of circular RNAs identifies hsa_circ_000455 as a new circular RNA biomarker for deep vein thrombosis
Microarray profile of circular RNAs identifies hsa_circ_000455 as a new circular RNA biomarker for deep vein thrombosis
复制标题
环状 RNA 的微阵列图谱将 hsa_circ_000455 鉴定为深静脉血栓形成的新环状 RNA 生物标志物
DOI：
10.1177/17085381211016150
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发表时间：
2021-05-22
期刊：
影响因子：
1.1
通讯作者：
Wang, Bing
中科院分区：
文献类型：
--
作者：
Lou, Zhenkai;Li, Xing;Wang, Bing
目的深静脉血栓形成是一种严重的静脉血栓栓塞症，可导致高死亡率和高致残率。在多种疾病中发现了环状RNA（circRNA）表达的改变。然而，circRNA在深静脉血栓形成中的功能仍然未知。方法选取深静脉血栓形成患者和健康对照者的血液标本，制备circRNA芯片，采用qPCR检测circRNA的表达。此外，进行GO/KEGG分析，并预测hsa_circ_RNA_000455靶向的miRNA-mRNA网络。结果芯片检测到303个circRNA在深静脉血栓形成中表达差异，其中83个表达上调，220个表达下调。实时荧光定量PCR检测结果与芯片结果一致。GO分析显示，深静脉血栓患者中前100个差异表达的circRNA与蛋白质转运、细胞质、三磷酸腺苷（ATP）结合密切相关。通过KEGG分析最显著富集的途径包括甲状腺素信号传导途径、内吞作用、癌症中的蛋白聚糖、Fc γ R介导的吞噬作用、粘着斑、胰岛素信号传导途径、p53信号传导途径、抗生素生物合成、上皮细胞的细菌侵袭和AMP活化蛋白激酶信号传导途径。选取hsa_circ_000455，通过circRNA-miRNA-mRNA网络分析hsa_circ_000455在深静脉血栓形成中的作用。因此，我们推测hsa_circRNA_000455/hsa-miR-22- 3 p/NLRP 3可能参与了深静脉血栓形成的发生。结论本研究首次提供了深静脉血栓形成中circRNA表达谱的信息，为hsa_circRNA_000455在深静脉血栓形成中的作用及机制提供了线索。
Objects Deep vein thrombosis is a type of severe venous thromboembolism that can result in high mortality and morbidity. The expression alternation of circular RNAs (circRNAs) has been found in various diseases. However, the function of circRNAs in deep vein thrombosis still remains unknown. Method The blood samples of deep vein thrombosis patients and health control were selected, circRNA microarray was performed, and qPCR was used to verify the expression of circRNAs. Also, GO/KEGG analysis was performed, and hsa_circ_RNA_000455-targeted miRNA–mRNA network was predicted. Result Here, we found that 303 circRNAs were differentially expressed in deep vein thrombosis using microarray, of which 83 circRNAs were upregulated and 220 circRNAs were downregulated. The expression of five circRNAs verified by quantitative real-time PCR was consistent with the result of microarray. GO analysis showed that the top 100 differentially expressed circRNAs in deep vein thrombosis patients were closely related to protein transport, cytoplasm, and Adenosine Triphosphate (ATP) binding. The most significantly enriched pathways by KEGG analysis included thyroid hormone-signaling pathway, endocytosis, proteoglycans in cancer, Fc gamma R-mediated phagocytosis, focal adhesion, insulin-signaling pathway, p53-signaling pathway, biosynthesis of antibiotics, bacterial invasion of epithelial cells, and AMP-activated protein kinase-signaling pathway. Then, hsa_circ_000455 was selected, and the function of hsa_circ_000455 in the pathogenesis of deep vein thrombosis was analyzed via circRNA–miRNA–mRNA network. We therefore hypothesized that hsa_circRNA_000455/hsa-miR-22-3p/NLRP3 may involve in the development of deep vein thrombosis. Conclusion This study provided valuable information on circRNA profile in deep vein thrombosis for the first time and gave clues on the possible role and mechanism of hsa_circRNA_000455 in deep vein thrombosis.