Antigens of Trypanosoma cruzi detected by different classes and subclasses of antibodies.
Antigens of Trypanosoma cruzi detected by different classes and subclasses of antibodies.
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通过不同类别和亚类的抗体检测到克氏锥虫抗原。
DOI：
10.1016/0035-9203(84)90237-2
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发表时间：
1984
影响因子：
2.2
通讯作者：
Tighe,L
中科院分区：
文献类型：
--
作者：
Araujo,FG;Heilman,B;Tighe,L
本文用间接免疫荧光抗体试验和蛋白转移技术研究了克氏锥虫感染小鼠后特异性IgM和IgG抗体亚类的出现动力学以及这些抗体所识别的无鞭毛体和上鞭毛体抗原。IgM和IgG 2抗体几乎同时出现，分别在感染后30天和40天达到高峰。感染后第150天，IgM抗体几乎检测不到，而IgG 2抗体仍处于高滴度。IgG 3和IgG 1分别在感染后20和30天首次检出，30和50天达高峰，150天仍能检出低滴度抗体。每种抗体的免疫荧光试验显示生物体荧光染色模式的差异，特别是无鞭毛体。这些差异在IgG 3抗体中最为显著。IgM或IgG 1荧光染色主要位于无鞭毛体的一极或两极; IgG 2荧光染色覆盖无鞭毛体或上鞭毛体的整个身体; IgG 3荧光染色以非常小的斑点形式覆盖两个阶段的生物体的整个身体。蛋白质印迹显示，每种抗体显然识别相同的抗原在两个epimastigote和无鞭毛体抗原制剂。外鞭毛体的90 Kd MW抗原以及无鞭毛体的MW 92 Kd和90 Kd的两种抗原被检查的每种抗体识别。
The kinetics of the appearance of specific IgM and of subclasses of IgG antibodies following infection of mice withTrypanosoma cruziand the antigens of amastigotes and epimastigotes recognized by these antibodies were investigated by using the indirect immunofluorescent antibody test and the protein transfer technique. IgM and IgG2antibodies were detected almost at the same time and peaked on day 30 and 40 of infection respectively. On day 150 of infection IgM antibodies were barely detectable whereas IgG2antibodies were still at a high titre. IgG3and IgG1antibodies were first detected on days 20 and 30, peaked on days 30 and 50 respectively, and were still detected at low titres on day 150 of infection. The immunofluorescent test with each antibody revealed differences in the patterns of the fluorescent staining of the organisms, particularly with amastigotes. These differences were most striking with IgG3antibodies. Fluorescent staining with IgM or IgG1was localized mostly on one or two poles of the amastigotes; with IgG2it was over the entire body of either amastigotes or epimastigotes; and with IgG3it was in the form of very small spots over the entire body of organisms of both stages. The Western blots revealed that each antibody apparently recognized the same antigens in both the epimastigote and amastigote antigen preparations. The 90 Kd MW antigen of epimastigotes as well as two antigens of MW 92 Kd and 90 Kd of amastigotes were recognized by each of the antibodies examined.