Expression of interferon type I and II, Mx and gammaIP genes in the kidney of Atlantic salmon, Salmo salar, is induced during smolting.
Expression of interferon type I and II, Mx and gammaIP genes in the kidney of Atlantic salmon, Salmo salar, is induced during smolting.
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大西洋鲑鱼 (Salmo salar) 肾脏中 I 型和 II 型干扰素、Mx 和 gammaIP 基因的表达在幼龄鲑鱼的幼龄期被诱导。
DOI：
10.1016/j.fsi.2006.10.010
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发表时间：
2007
影响因子：
4.7
通讯作者：
A. E. Ellis
中科院分区：
文献类型：
--
作者：
Basanta Kumar Das;Bertrand Collet;Michael Snow;A. E. Ellis
采用qRT-PCR监测了大西洋鲑鱼种群在幼鱼孵化和海水转移之前和期间肾组织中I型干扰素（IFNα）和II型干扰素（IFNγ）基因及其两种诱导基因Mx和γIP的表达。在10月份通过光周期操作诱导了小鲑鱼的生长过程，并在12月份将小鲑鱼转移到海水中。在10月份延长光照期之前，鱼显示出所测定的基因的极低水平表达。然而，在延长光照后即刻和转移到海水中1周后，90条鱼中有26条鱼的IFNα、Mx和γIP表达上调。两种鱼在海水转移前24天显示出最高水平。11条鱼显示IFNγ的表达升高，但与二龄鲑或海水转移或其他基因的表达没有明显的关联。在采样期结束时，用标准病毒学方法对30条鱼进行了测试，发现没有病毒。结果表明，在幼鱼孵化过程中，IFNα和Mx mRNA持续表达。在接近转移到海水中的时间表达Mx的那些个体预期在海水转移后的最长时间内在组织中具有高水平的抗病毒Mx蛋白。这可以提供一种先天的防御，以抵御小鲑鱼进入海洋环境时可能首次遇到的病毒病原体。那些在小鲑鱼早期表达Mx的个体可能更有可能在小鲑鱼后期发展IPN或其他病毒性疾病。
The expression in kidney tissue of interferon type I (IFNα) and type II (IFNγ) genes and two of their inducible genes, Mx and γIP were monitored, using qRT–PCR, in a population of Atlantic salmon prior to and over the period of smolting and sea water transfer. The smolting process was induced by photoperiod manipulation in October and smolts were transferred to sea water in December. Prior to extending the light period in October, the fish showed extremely low level expression of the genes assayed. However, immediately on extending the light and up until 1week after transfer to sea water, 26 of the 90 fish sampled showed up-regulated expression for IFNα, Mx and γIP. The highest levels were shown by two fish on the 2days prior to sea water transfer. Eleven fish displayed elevated expression of IFNγ but there was no apparent association with smolting or sea water transfer or expression of the other genes. At the end of the sampling period, 30 fish were tested by standard virological methods and found to be virus free. The results indicate that during the smolting process, Atlantic salmon consititutively express IFNα and Mx mRNA. Those individuals which express Mx close to the time of transfer to sea water would be expected to have high levels of the anti-viral Mx protein in tissues for the longest time after sea water transfer. This could provide an innate defence against viral pathogens which post-smolts may encounter for the first time on entering the marine environment. Those individuals which express Mx early in the smolting process may be more at risk of developing IPN or other viral diseases as post-smolts.