The influence of deep hypothermia on inflammatory status, tissue hypoxia and endocrine function of adipose tissue during cardiac surgery
The influence of deep hypothermia on inflammatory status, tissue hypoxia and endocrine function of adipose tissue during cardiac surgery
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DOI：
10.1016/j.cryobiol.2014.02.007
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发表时间：
2014-04-01
期刊：
影响因子：
2.7
通讯作者：
Haluzik, Martin
中科院分区：
文献类型：
--
作者：
Drapalova, Jana;Kopecky, Petr;Haluzik, Martin
手术中脂肪组织内分泌功能的改变，如促炎细胞因子的过度产生，可显著改变手术后的代谢反应，恶化手术结果和患者的预后。治疗性低温已被用于预防与围手术期缺血和低灌流有关的损伤。本研究旨在探讨深低温对全身和局部炎症、脂肪组织缺氧和脂肪细胞因子产生的影响。我们比较了血清促炎标志物(CRP、IL-6、IL-8、sIL-2R、sTNFRI、PCT)的浓度以及参与炎症反应(IL-6、TNF-α、MCP-1、MIF)和低氧和氧化应激适应(HIF1-α、MT3、GLUT1、IRS1、GPX1、bcl2)的特定基因在皮下和内脏脂肪组织以及体外培养的低温期心脏手术患者脂肪细胞中的表达。深低温显著延迟了手术相关全身炎症反应的发生。研究基因的相对基因表达在低温期间没有改变，但在手术结束时10个基因中的6个(IL-6、MCP-1、TNF-α、HIF1-α、GLUT1、GPX1)发生了显著变化。我们的结果表明，深低温可以抑制全身炎症反应的发展，延迟局部脂肪组织炎症的发生，从而可能对心脏手术患者的促炎和低氧相关因子的过度表达具有保护作用。(C)2014 Elsevier Inc.保留所有权利。
Changes in endocrine function of adipose tissue during surgery, such as excessive production of proinflammatory cytokines, can significantly alter metabolic response to surgery and worsen its outcomes and prognosis of patients. Therapeutic hypothermia has been used to prevent damage connected with perioperative ischemia and hypoperfusion. The aim of our study was to explore the influence of deep hypothermia on systemic and local inflafnmation, adipose tissue hypoxia and adipocytokine production. We compared serum concentrations of proinflammatory markers (CRP, IL-6, IL-8, sIL-2R, sTNFRI, PCT) and mRNA expression of selected genes involved in inflammatory reactions (IL-6, TNF-alpha, MCP-1, MIF) and adaptation to hypoxia and oxidative stress (HIF1-alpha, MT3, GLUT1, IRS1, GPX1, BCL-2) in subcutaneous and visceral adipose tissue and in isolated adipocytes of patients undergoing cardiosurgical operation with hypothermic period. Deep hypothermia significantly delayed the onset of surgery-related systemic inflammatory response. The relative gene expression of the studied genes was not altered during the hypothermic period, but was significantly changed in six out of ten studied genes (IL-6, MCP-1, TNF-alpha, HIF1-alpha, GLUT1, GPX1) at the end of surgery. Our results show that deep hypothermia suppresses the development of systemic inflammatory response, delays the onset of local adipose tissue inflammation and thus may protect against excessive expression of proinflammatory and hypoxia-related factors in patients undergoing elective cardiac surgery procedure. (C) 2014 Elsevier Inc. All rights reserved.