1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window
1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window
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DOI：
10.1007/s12274-014-0549-1
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发表时间：
2015-02
期刊：
影响因子：
9.9
通讯作者：
I. Villa;A. Vedda;Irene Xochilt Cantarelli;M. Pedroni;F. Piccinelli;Marco Bettinelli;A. Speghini;
中科院分区：
文献类型：
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作者：
I. Villa;A. Vedda;Irene Xochilt Cantarelli;M. Pedroni;F. Piccinelli;Marco Bettinelli;A. Speghini;
高对比度、深层组织、体内荧光生物医学成像的新方法基于在所谓的第二生物窗口（1,000–1,400 nm）中工作的红外发射纳米颗粒。由于组织在此特定光谱范围内部分透明，因此可以获取高分辨率的深层组织图像。此外，低能量（红外）光子的光学激发也导致自发荧光对体内图像的贡献急剧减少。然而，正如这里所证明的，仅在这个生物窗口中工作并不能确保完全消除自发荧光，因为样本的饮食显示出显着的红外荧光，波长可达 1,100 nm。在这项工作中，我们展示了如何使用嵌入 SrF2 纳米颗粒中的 Nd3+ 离子的 1,340 nm 发射带来产生无自发荧光、高对比度体内荧光图像。还证明，完全消除与食品相关的红外自发荧光对于开展可靠的生物分布研究至关重要。
Novel approaches for high contrast, deep tissue,in vivofluorescence biomedical imaging are based on infrared-emitting nanoparticles working in the so-called second biological window (1,000–1,400 nm). This allows for the acquisition of high resolution, deep tissue images due to the partial transparency of tissues in this particular spectral range. In addition, the optical excitation with low energy (infrared) photons also leads to a drastic reduction in the contribution of autofluorescence to thein vivoimage. Nevertheless, as is demonstrated here, working solely in this biological window does not ensure a complete removal of autofluorescence as the specimen’s diet shows a remarkable infrared fluorescence that extends up to 1,100 nm. In this work, we show how the 1,340 nm emission band of Nd3+ions embedded in SrF2nanoparticles can be used to produce autofluorescence free, high contrastin vivofluorescence images. It is also demonstrated that the complete removal of the food-related infrared autofluorescence is imperative for the development of reliable biodistribution studies.