Twenty-eight-day bed rest with hypercortisolemia induces peripheral insulin resistance and increases intramuscular triglycerides.
Twenty-eight-day bed rest with hypercortisolemia induces peripheral insulin resistance and increases intramuscular triglycerides.
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DOI：
10.1016/j.metabol.2009.09.014
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发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Ferrando A
中科院分区：
文献类型：
--
作者：
Cree MG;Paddon-Jones D;Newcomer BR;Ronsen O;Aarsland A;Wolfe RR;Ferrando A
太空飞行对人类来说是一种独特的生理挑战，它改变了荷尔蒙分布和组织胰岛素敏感性。在这些荷尔蒙变化中，高皮质醇血症和胰岛素不敏感被认为对航天运动中的肌肉质量和功能产生负面影响。由于胰岛素敏感性影响肌肉甘油三酯的积累，我们研究了高皮质醇血症和不活动时的这种关系。6名年轻的健康志愿者卧床休息28天。为了模拟航天飞行中观察到的应激反应，通过口服氢化可的松诱导和维持高皮质醇血症(20-24 mg/dL)。在卧床第1天和第28天，用局部(股动脉胰岛素输注)2级高胰岛素-正常血糖钳夹(阶段1：35微U/分/毫升腿；阶段2：70微U/分/毫升腿)评估整个小腿的胰岛素敏感性。用磁共振波谱测定肌内脂肪含量。卧床休息后，胰岛素敏感性下降，通过高胰岛素血症时的葡萄糖摄取来评估(从9.1±1.3mg.Kg.腿/分钟降至5.2±0.7mg.kg.leg/分钟(P=0.015))。肌内甘油三酯由0.077±0.011增至0.136±0.018(脂肪信号面积/标准信号面积；P=0.009)。28d肌内脂肪含量与葡萄糖摄取量呈正相关(R=−0.85；P=0.035)。这些数据表明，在以前健康的受试者中，肌肉不活动和高皮质醇血症与肌肉内甘油三酯和骨骼肌胰岛素抵抗的增加有关。
Spaceflight represents a unique physiological challenge to humans, altering hormonal profiles and tissue insulin sensitivity. Among these hormonal alterations, hypercortisolemia and insulin insensitivity are thought to negatively affect muscle mass and function with spaceflight. As insulin sensitivity influences the accumulation of muscle triglycerides, we examined this relationship during hypercortisolemia and inactivity. Six young healthy volunteers were confined to bed rest for 28 days. To mimic the stress response observed during spaceflight, hypercortisolemia (20–24mg/dL) was induced and maintained by oral ingestion of hydrocortisone. On days 1 and 28 of bed rest, insulin sensitivity across the leg was assessed with a local (femoral arterial insulin infusion) 2 stage hyperinsulinemic-euglycemic clamp (stage 1: 35 µU/min/ml leg; stage 2: 70 µU/min/ml leg). Intramuscular lipid was measured with magnetic resonance spectroscopy. Following bed rest, there was a decrease in insulin sensitivity, as assessed by glucose uptake during hyperinsulinemia (from 9.1±1.3 (mean ± SEM) mg/kg.leg/min to 5.2±0.7 mg/kg.leg/min (P=0.015)). Intramuscular triglyceride increased from 0.077±0.011 to 0.136±0.018 (signal area of fat/signal area of standard; P=0.009). Intramuscular lipid content correlated with the glucose uptake at day 28, (R= −0.85; P=0.035). These data demonstrate that muscular inactivity and hypercortisolemia are associated with an increase in intramuscular triglyceride and skeletal muscle insulin resistance in previously healthy subjects.
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影响因子：
4.5
作者：
Cree MG;Newcomer BR;Herndon DN;Qian T;Sun D;Morio B;Zwetsloot JJ;Dohm GL;Fram RY;Mlcak RP;Aarsland A;Wolfe RR
通讯作者：
Wolfe RR
影响因子：
7
作者：
Hulver, MW;Dohm, GL
通讯作者：
Dohm, GL
影响因子：
--
作者：
CARTERSU, C;OKAMOTO, K
通讯作者：
OKAMOTO, K
DOI：
10.1152/ajpendo.00397.2002
发表时间：
2003-05-01
影响因子：
5.1
作者：
Paddon-Jones, D;Sheffield-Moore, M;Ferrando, AA
通讯作者：
Ferrando, AA
影响因子：
3.3
作者：
KLOSE, U
通讯作者：
KLOSE, U