Three-layered structure shared between Lewy bodies and Lewy neurites - Three-dimensional reconstruction of triple-labeled sections
Three-layered structure shared between Lewy bodies and Lewy neurites - Three-dimensional reconstruction of triple-labeled sections
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DOI：
10.1111/j.1750-3639.2008.00140.x
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发表时间：
2008-07-01
期刊：
影响因子：
6.4
通讯作者：
Mizusawa, Hidehiro
中科院分区：
文献类型：
--
作者：
Kanazawa, Toshiro;Uchihara, Toshiki;Mizusawa, Hidehiro
路易体（LBs）和路易神经突（LNs）是帕金森病（PD）的标志。虽然经常共存的lb和ln有一些共同的组织学特征，但在常规的二维观察下，它们的外观却有很大的不同。为了阐明这些明显不同的结构（LBs和LNs）在形成过程中是如何相关的，我们对PD患者死后的脑干组织进行了三维观察。对60 μ m厚的浮片进行α -突触核蛋白（α S）、泛素（Ub）和神经丝（NF）的免疫荧光标记。用软件对序列共聚焦图像进行重建。lb的外部三维构型，双标记α S和NF，与LNs表现出频繁的连续性（70%）。在内部，α S和Ub形成三维同心内层，NF围绕这些内层。这种层状结构在球形lb、棒状lb和甚至卷曲形式的lb /LNs中都存在。此外，每一层都表现出不间断的连续性，即使是在卷曲形式和其与球形lb的连接处。这种三层结构在各种Lewy病理中共享，并且在三维基础上具有层状连续性，支持了LNs进化为lb的假设。除了从苍白体到lb的演化，从lb到lb的结构演化可能为α S矿床的变异性及其相互关系提供了另一种解释。
Lewy bodies (LBs) and Lewy neurites (LNs) are the hallmarks of Parkinson's disease (PD). Although LBs and LNs, frequently coexistent, share some histological properties, their appearances are quite different under conventional two-dimensional observation. In order to clarify how these apparently different structures (LBs and LNs) are related during their formation, we performed three-dimensional observation on post-mortem brainstem tissues with PD. Sixty-mu m thick floating sections were multi-immunofluorolabeled for alpha-synuclein (alpha S), ubiquitin (Ub) and neurofilament (NF). Serial confocal images were reconstructed with software. External three-dimensional configuration of LBs, double-labeled for alpha S and NF, exhibited frequent continuity with LNs (70%). Internally, alpha S and Ub formed the three-dimensional concentric inner layers and NF rimmed these inner layers. This layered structure was shared among spherical LBs, rod-shaped LNs and even convoluted forms of LBs/LNs. Furthermore, each layer exhibited continuity without interruption even in the convoluted form and around its junction to spherical LBs. This three-layered structure shared among various Lewy pathologies and their layered continuity on three-dimensional basis favor the hypothesis that LNs evolve into LBs. Besides progression from pale bodies to LBs, structural evolution from LNs into LBs may provide an alternative explanation for the variability of alpha S deposits and their interrelation.