Identification of Genes Induced in Response to Low-Temperature Treatment in Tea Leaves
Identification of Genes Induced in Response to Low-Temperature Treatment in Tea Leaves
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DOI：
10.1007/s11105-008-0079-7
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发表时间：
2009-09
影响因子：
2.1
通讯作者：
Lei Wang;Xianwen Li;Qing Zhao;S. Jing;Shi Chen;Hongyu Yuan
中科院分区：
文献类型：
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作者：
Lei Wang;Xianwen Li;Qing Zhao;S. Jing;Shi Chen;Hongyu Yuan
利用抑制消减杂交技术对茶树（Camellia sinensis（L.）O. Kuntze），一种阔叶、木质万年青物种。鉴定了10个茶树叶片低温诱导的互补DNA（cDNA）克隆。这些转录本可以分为三个功能组。三个转录本编码转录因子。其中，Cs-COR 375含有一个开放阅读框架，编码一个具有两个EPF型锌指的锌指蛋白，而Cs-COR 041和Cs-COR 178对应于CBF样基因。Cs-COR 044、Cs-COR 196和Cs-COR 213 cDNA的预测产物均为细胞结构蛋白，Cs-COR 044编码一个86个氨基酸的蛋白质，其N端为疏水区，C端为亲水区，富含甘氨酸和脯氨酸。Cs-COR 196和Cs-COR 213编码两个早期光诱导蛋白，它们是一类核编码的类囊体膜蛋白，在各种胁迫下积累。预测的蛋白质编码的其余四个转录本是pupillary参与初级和次级代谢。Cs-COR 018编码的产物是β-淀粉酶，参与多种植物的低温驯化，Cs-COR 126编码查尔酮合成酶，Cs-COR 054和Cs-COR 113编码两种不同的黄酮醇脱氢酶。
A suppression subtractive hybridization analysis approach was undertaken to identify genes involved in cold acclimation of tea (Camellia sinensis(L.) O. Kuntze), a broad-leaved, woody evergreen species. A total of ten complementary DNA (cDNA) clones, induced by low temperature in tea leaves, were identified. These transcripts could be classified into three functional groups. Three transcripts encoded transcriptional factors. Among these,Cs-COR375contained a putative open reading frame encoding a zinc-finger protein with two EPF-type zinc fingers, whileCs-COR041andCs-COR178were corresponded toCBF-like genes. The predicted products ofCs-COR044,Cs-COR196, andCs-COR213cDNAs were cell structure proteins.Cs-COR044encoded a putative 86 amino acid protein consisting of a hydrophobic N-terminal half and a hydrophilic C-terminal half relatively rich in glycine and proline. The protein was predicted to be exported to the apoplast and likely to be localized in the cell wall.Cs-COR196andCs-COR213encoded for two putative early light-induced proteins, a group of nuclear-encoded thylakoid membrane proteins accumulating in response to various stresses. The predicted proteins encoded by the remaining four transcripts were putatively involved in primary and secondary metabolism. The product encoded byCs-COR018was β-amylase, involved in cold acclimation of various plant species.Cs-COR126coded for chalcone synthase, whileCs-COR054andCs-COR113coded for two different flavonol synthases.