IgG and IgE avidity characteristics of peanut allergic individuals
IgG and IgE avidity characteristics of peanut allergic individuals
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DOI：
10.1111/j.1399-3038.2007.00542.x
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发表时间：
2007-11-01
影响因子：
4.4
通讯作者：
Hourihane, Jonathan O'Brien
中科院分区：
文献类型：
--
作者：
El-Khouly, Fatima;Lewis, Stella Anne;Hourihane, Jonathan O'Brien
抗体亲合力在变态反应中的作用知之甚少，并且没有现有文献描述食物变态反应中的抗体亲合力。本研究的主要目的是调查总花生蛋白提取物（TPPE）和纯化的Ara h 2在一组特征良好的花生过敏个体的IgE和IgG亲和力。40名花生过敏患者接受了双盲安慰剂对照的低剂量花生激发试验，在此期间对患者花生过敏的严重程度进行评分。血清花生特异性IgE（psIgE）和IgG（psIgG）浓度测定37个人和相同的抗体的亲和力TPPE和纯化的阿糖胞苷h 2使用硫氰酸盐ELISA法测定。IgE和IgG对Ara h 2的亲合力与攻击评分呈弱正相关[分别为r = 0.459（p = 0.012）和r = 0.486（p = 0.003）]。IgE对TPPE的亲合力与皮肤点刺试验（SPT）结果呈弱正相关，r = 0.467（p = 0.004），总IgE：psIgE比值与激发评分呈负相关，r = -0.561（p < 0.001）。IgE抗体亲合力：IgG抗体亲合力比值与攻毒评分或SPT之间无显著相关性。这是第一次描述的IgE和IgG亲合力在花生过敏，它似乎是IgE和IgG抗体的亲合力纯化的阿糖胞苷h 2弱相关的花生过敏的严重程度（如由挑战评分测量）。
The role of antibody avidity in allergy is poorly understood and there is no existing literature describing antibody avidity in food allergy. The main aim of this study was to investigate IgE and IgG avidity to a total peanut protein extract (TPPE) and purified Ara h 2 in a group of well-characterized peanut allergic individuals. Forty peanut allergic patients underwent a double-blind placebo-controlled low-dose peanut challenge, during which the severity of the patients' peanut allergy was scored. Serum peanut-specific IgE (psIgE) and IgG (psIgG) concentrations were measured for 37 individuals and the avidities of the same antibodies to a TPPE and purified Ara h 2 were determined using a thiocyanate ELISA method. Both IgE and IgG avidity to Ara h 2 showed weak positive correlations with challenge score [r = 0.459 (p = 0.012) and r = 0.486 (p = 0.003), respectively]. IgE avidity to TPPE showed a weak positive correlation with skin prick test results (SPT), r = 0.467 (p = 0.004) and there was an inverse relationship between the ratio of total IgE:psIgE and challenge score r = -0.561 (p < 0.001). No significant relationship was found between the ratios of IgE avidity:IgG avidity and challenge score or SPT. This is the first description of IgE and IgG avidity in peanut allergy, and it appears that the avidities of IgE and IgG antibodies to purified Ara h 2 are weakly related to the severity of peanut allergy (as measured by a challenge score).