EVOLUTION AND PHYLOGENY OF WOLBACHIA - REPRODUCTIVE PARASITES OF ARTHROPODS
EVOLUTION AND PHYLOGENY OF WOLBACHIA - REPRODUCTIVE PARASITES OF ARTHROPODS
复制标题
DOI：
10.1098/rspb.1995.0117
复制
发表时间：
1995-07-22
影响因子：
4.7
通讯作者：
GUO, LR
中科院分区：
文献类型：
--
作者：
WERREN, JH;ZHANG, W;GUO, LR
沃尔巴克氏体是一种存在于许多节肢动物生殖组织中的细胞质遗传细菌。这些细菌与宿主的生殖改变有关，包括孤雌生殖、生殖不相容性和雌性化。使用来自ftsZ（一种快速进化的细菌细胞周期基因）的DNA序列进行了精细的系统发育分析。测定了来自31种不同昆虫和一种等足类的38种不同沃尔巴克氏体菌株的ftsZ序列。结果表明：（1）沃尔巴克氏体存在两个主要的分支（A和B），根据同义替换率，它们在距今5800 - 6700万年前发生了分化;（2）ftsZ和16 SrDNA同源性基本一致，表明这两个分支代表了一个细菌菌株（而不是简单的基因）重组;然而，A和B分裂细菌之间重组的可能实例可能发生在等足类动物中存在的雌性化沃尔巴克氏体中;（iii）沃尔巴克氏体在昆虫类群之间，包括不同的昆虫目之间发生了广泛的水平传播;特别是一个菌株（指定为Adm）显示了近期广泛的水平传播;（iv）寄生蜂（Nasonia）体内的沃尔巴克氏体与其苍蝇宿主之间存在关联（Protocalliphora），表明这些物种之间的细菌交换;（v）孤雌生殖诱导在沃尔巴克氏体中进化了几次;以及（vi）一些昆虫甚至在个体昆虫中也感染了一种以上的沃尔巴克氏体菌株。
Wolbachia are cytoplasmically inherited bacteria found in reproductive tissues of many arthropod species. These bacteria are associated with reproductive alterations in their hosts, including parthenogenesis, reproductive incompatibility and feminization. A fine-scale phylogenetic analysis was done using DNA sequences from ftsZ, a rapidly evolving bacterial cell-cycle gene. ftsZ sequences were determined for 38 different Wolbachia strains from 31 different species of insects and one isopod. The following results were found: (i) there are two major division of Wolbachia (A and B) which diverged 58-67 millions years before present based upon synonymous substitution rates; (ii) a general concordance is found between the ftsZ and 16S rDNA phylogenies, indicating that these represent bacterial strain (rather than simply gene) phylogenies; however, a possible example of recombination between A and B division bacteria may have occurred in the feminizing- Wolbachia present in an isopod; (iii) extensive horizontal transmission of Wolbachia has occurred between insect taxa, including different insect orders; one strain in particular (designated Adm) shows extensive recent horizontal transmission; (iv) there is an association between the Wolbachia found in a parasitic wasp (Nasonia) and its fly host (Protocalliphora), suggesting exchange of bacteria between these species; (v) parthenogenesis induction has evolved several times among the Wolbachia; and (vi) some insects harbour infections with more than one Wolbachia strain, even within individual insects.