Specific combinations of SR proteins associate with single pre-messenger RNAs in vivo and contribute different functions.
Specific combinations of SR proteins associate with single pre-messenger RNAs in vivo and contribute different functions.
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DOI：
10.1083/jcb.200806156
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发表时间：
2009-02-23
期刊：
影响因子：
--
通讯作者：
Wieslander L
中科院分区：
文献类型：
--
作者：
Björk P;Jin S;Zhao J;Singh OP;Persson JO;Hellman U;Wieslander L
富含丝氨酸/丝氨酸（SR）的蛋白质是信使RNA（mRNA）加工、输出、监视和翻译所必需的。我们发现，在摇蚊tentans，新生的成绩单与多种类型的SR蛋白在特定的组合。选择性剪接因子（ASF）/SF 2、SC 35、9 G8和hrp 45/SRp 55都优先存在于Balbiani环（BR）前信使核糖核蛋白（mRNP）中，当内含子出现在前mRNA中时，并且当共转录剪接发生时。然而，hrp 45/SRp 55与ASF/SF 2、SC 35和9 G8相比在pre-mRNP中沿着基因分布不同，表明功能差异。所有四种SR蛋白在输出到细胞质期间与BR mRNP相关联。对SC 35的干扰表明SC 35对剪接、转录和3′端加工的协调以及核质输出都很重要。ASF/SF 2与多核糖体相关，而SC 35、9 G8和hrp 45/SRp 55与单核糖体共沉积。因此，单个内源性pre-mRNP/mRNP在转录过程中结合多种类型的SR蛋白，并且这些SR蛋白伴随mRNA并在体内基因表达途径中发挥不同的作用。
Serine/arginine-rich (SR) proteins are required for messenger RNA (mRNA) processing, export, surveillance, and translation. We show that in Chironomus tentans, nascent transcripts associate with multiple types of SR proteins in specific combinations. Alternative splicing factor (ASF)/SF2, SC35, 9G8, and hrp45/SRp55 are all present in Balbiani ring (BR) pre-messenger ribonucleoproteins (mRNPs) preferentially when introns appear in the pre-mRNA and when cotranscriptional splicing takes place. However, hrp45/SRp55 is distributed differently in the pre-mRNPs along the gene compared with ASF/SF2, SC35, and 9G8, suggesting functional differences. All four SR proteins are associated with the BR mRNPs during export to the cytoplasm. Interference with SC35 indicates that SC35 is important for the coordination of splicing, transcription, and 3′ end processing and also for nucleocytoplasmic export. ASF/SF2 is associated with polyribosomes, whereas SC35, 9G8, and hrp45/SRp55 cosediment with monoribosomes. Thus, individual endogenous pre-mRNPs/mRNPs bind multiple types of SR proteins during transcription, and these SR proteins accompany the mRNA and play different roles during the gene expression pathway in vivo.
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DOI：
10.1083/jcb.200407173
发表时间：
2005-03-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kiesler E;Hase ME;Brodin D;Visa N
通讯作者：
Visa N
影响因子：
11.4
作者：
Bedard, Kristin M.;Daijogo, Sarah;Semler, Bert L.
通讯作者：
Semler, Bert L.
影响因子：
10.5
作者：
AlzhanovaEricsson, AT;Sun, X;Daneholt, B
通讯作者：
Daneholt, B
影响因子：
4
作者：
Björk, P;Baurén, G;Wieslander, L
通讯作者：
Wieslander, L
影响因子：
14.9
作者：
CALZONE, FJ;ANGERER, RC;GOROVSKY, MA
通讯作者：
GOROVSKY, MA