Effects of isolated rheumatoid synovial cells on cartilage degradation in vitro.
Effects of isolated rheumatoid synovial cells on cartilage degradation in vitro.
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分离的类风湿滑膜细胞对体外软骨降解的影响。
DOI：
10.1002/jor.1100080306
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Thornhill,TS
中科院分区：
文献类型：
--
作者：
Keller,K;Shortkroff,S;Sledge,CB;Thornhill,TS
与软骨共培养的类风湿性滑膜已被证明可以释放一种因子，刺激软骨基质中糖胺聚糖（GAG）的消耗。人类类风湿滑膜被酶解，分离的细胞受到各种机械和免疫处理。制备并分析了滑膜细胞条件培养基（SCCM）刺激GAG耗竭的能力。从分离的滑膜细胞浓度增加制备的SCCM以剂量依赖的方式显示出软骨降解活性。这种活性表现为白细胞介素- 1样，主要存在于黏附细胞群中，其中滑膜巨噬细胞保留了显著的降解能力。单独的T细胞被发现对软骨没有直接的降解作用，但它们的存在似乎增强了附着细胞的反应。本文描述的技术提供了一个定量模型，用于检查滑膜中的降解因子以及促进其释放的细胞相互作用。
Rheumatoid synovium in coculture with cartilage has been shown to release a factor(s) that stimulates the depletion of glycosaminoglycans (GAG) from cartilage matrix. Human rheumatoid synovium was enzymatically disaggregated and the isolated cells were subjected to a variety of mechanical and immunological treatments. Synovial cell conditioned media (SCCM) were prepared and analyzed for their ability to stimulate GAG depletion. SCCM prepared from increasing concentrations of isolated synovial cells demonstrated cartilage degradative activity in a dose‐dependent manner. This activity was characterized as interleukin‐l like and was found mostly within the adherent cell population where the synovial macrophages retained significant degradative ability. T cells alone were found to have no direct degradative effect on cartilage, but their presence appeared to augment the response of the adherent cells. The techiques described here provide a quantitative model for examining the degradative factors from synovium as well as the cellular interactions that promote their release.