Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota
Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota
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DOI：
10.1111/j.1574-695x.2009.00553.x
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发表时间：
2009-06-01
影响因子：
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通讯作者：
Nakayama, Jiro
中科院分区：
文献类型：
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作者：
Tanaka, Shigemitsu;Kobayashi, Takako;Nakayama, Jiro
在26名婴儿中监测了出生后早期抗生素暴露对肠道微生物群发育的影响，其中包括5名在出生后前4天口服广谱抗生素的抗生素治疗（AT）受试者和3名剖腹产（CD）受试者，其母亲在同一时期静脉注射类似类型的抗生素。前5天每天分析粪便细菌组成，前2个月每月分析。AT受试者中的末端限制性片段长度多态性显示出较少的多样性，与对照组相比，某些细菌群的定殖减弱，特别是在第一周内双歧杆菌和肠球菌的不寻常定殖（AF，n=18）。实时定量PCR显示AT组肠球菌过度生长（第3天，P=0.01;第5天，P=0.003;第1个月，P=0.01），双歧杆菌生长停滞（第3天，P=0.03）。此外，1个月后，AT组的肠杆菌科细菌数量明显高于AF组（1个月，P=0.02; 2个月，P=0.02）。CD婴儿在发育中的微生物群中持续了类似的变化，尽管相对较弱。这些结果表明，生命开始时的抗生素暴露极大地影响了新生儿肠道微生物群的发育。
The influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota was monitored in 26 infants including five antibiotic-treated (AT) subjects orally administered a broad-spectrum antibiotic for the first 4 days of life and three caesarean-delivered (CD) subjects whose mothers were intravenously injected by the similar type of antibiotics in the same period. The faecal bacterial composition was analysed daily for the first 5 days and monthly for the first 2 months. Terminal restriction fragment length polymorphisms in the AT subjects showed less diversity with the attenuation of the colonization of some bacterial groups, especially in Bifidobacterium and unusual colonization of Enterococcus in the first week than the control antibiotic-free infants (AF, n=18). Quantitative real-time PCR showed overgrowth of enterococci (day 3, P=0.01; day 5, P=0.003; month 1, P=0.01) and arrested growth of Bifidobacterium (day 3, P=0.03) in the AT group. Furthermore, after 1 month, the Enterobacteriaceae population was markedly higher in the AT group than in the AF group (month 1, P=0.02; month 2, P=0.02). CD infants sustained similar, although relatively weaker, alteration in the developing microbiota. These results indicate that antibiotic exposure at the beginning of life greatly influences the development of neonatal intestinal microbiota.