PRIMARY, SECONDARY AND TERTIARY MYOTUBES IN DEVELOPING SKELETAL-MUSCLE - A NEW APPROACH TO THE ANALYSIS OF HUMAN MYOGENESIS
PRIMARY, SECONDARY AND TERTIARY MYOTUBES IN DEVELOPING SKELETAL-MUSCLE - A NEW APPROACH TO THE ANALYSIS OF HUMAN MYOGENESIS
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DOI：
10.1016/0022-510x(87)90181-x
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发表时间：
1987-10-01
影响因子：
4.4
通讯作者：
FITZSIMONS, RB
中科院分区：
文献类型：
--
作者：
DRAEGER, A;WEEDS, AG;FITZSIMONS, RB
肌球蛋白的单克隆抗体已被用来描述和定义的外观和成熟的3种不同类别的肌管在发展中的人股四头肌。使用了5种单克隆抗体：（i）抗人慢肌球蛋白重链的MA B A;（ii）抗大多数成人2型肌纤维中存在的肌球蛋白重链的MA B B;（iii）抗所有成熟和未成熟2型肌纤维中存在的肌球蛋白重链的MA B C;（iv）与MA B C具有相似反应性的MA B D;（v）抗人胚胎肌球蛋白的MA B E。这些抗体中的两种（A和B）的组合使用使得能够可靠地早期鉴定肌管的3类（初级、次级、三级）中的每一类，其在肌发生期间顺序出现。我们的研究结果表明，诱导缓慢肌球蛋白重链的合成是一个双相现象，在发育中的人骨骼肌。慢肌球蛋白重链存在于所有最早（9周妊娠）的初级肌管，但没有检测到二级或三级肌管，直到约29周妊娠。胎儿肌肉发育的每个阶段都有一个特征性的免疫细胞化学模式，该模式揭示了肌球蛋白ATP酶组织化学上不明显的细胞异质性。肌球蛋白免疫组化可用于评估胎儿的胎龄。基于3种不同肌管的分离编程，提出了一种新的骨骼肌发育解释。这可能是重要的，在患病肌肉的发育异常或再生存在的分析。
Monoclonal antibodies to myosins have been used to describe and define the appearance and maturation of 3 different classes of myotube in developing human quadriceps muscle. Five monoclonal antibodies wre used: (i) MAb A against human slow myosin heavy chain; (ii) MAb B against a myosin heavy chain present in most adult Type 2 fibres; (iii) MAb C against myosin heavy chain present in all mature and immature Type 2 fibres; (iv) MAb D, with similar reactivity to MAb C; (v) MAb E against human embryonic myosin. The combined use of two of these antibodies (A and B) enables the confident early identification of each of 3 classes (primary, secondary, tertiary) of myotubules, which appear sequentially during myogenesis. Our results show that induction of slow myosin heavy chain synthesis is a biphasic phenomenon in developing human skeletal muscle. Slow myosin heavy chain was present in all the earliest (9 weeks gestation) primary myotubes, but was not detected in secondary or tertiary myotubes until about 29 weeks gestation. Each stage of fetal muscle development has a characteristic immunocytochemical pattern which reveals cellular heterogeneity not evident on myosin ATPase histochemistry. Myosin immunochemistry may usefully be applied to assess the gestational age of fetuses. A new interpretation of human skeletal muscle development is proposed, based on the separate programming of 3 different kinds of myotube. This may be important in the analysis of diseased muscle in which developmental abnormalities or regeneration are present.