Skeletal muscle fiber cross-sectional area: effects of freezing procedures.
Skeletal muscle fiber cross-sectional area: effects of freezing procedures.
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DOI：
10.1159/000147798
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
R. R. Roy-R.;D. Pierotti;V. Edgerton
中科院分区：
文献类型：
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作者：
R. R. Roy-R.;D. Pierotti;V. Edgerton
单个纤维的横截面积（CSA）是衡量骨骼肌可塑性的重要指标。为了研究不同冷冻程序对CSA测量的影响，在将猫胫骨前肌快速冷冻到固定的生理长度（同侧，冷冻长度）后，测定了类型识别纤维的CSA，并将其与在组织准备过程中允许纤维自由缩短的肌肉中部快速冷冻后的纤维CSA进行了比较（对侧，冷冻为块）。每一种纤维类型的平均CSA在长时间冻结的肌肉中明显小于在肌肉的深层（接近骨骼）和浅层（远离骨骼）区域冻结的肌肉，除了浅层区域的缓慢氧化（SO）纤维。与快速氧化糖酵解纤维和快速糖酵解纤维相比，深层（分别为41%、47%和56%）和表层（分别为20%、36%和48%）的平均纤维CSA百分比差异较小。此外，两种冷冻过程中快纤维的平均csa差异在深层比表层大约10%。纤维类型的差异响应可能至少部分地与纤维的结构特征有关。这些数据表明，在确定单个骨骼肌纤维的CSA以及肌肉单位单位CSA的特定张力时，用于制备肌肉组织的冷冻程序是一个重要的考虑因素。
The cross-sectional area (CSA) of individual fibers is an important measure of skeletal muscle plasticity. To investigate the effects of different freezing procedures on CSA measurements, the CSA of type-identified fibers in the cat tibialis anterior were determined following quick-freezing the muscle at a fixed physiological length (ipsilateral, frozen at length) and compared to the fiber CSA following quick-freezing a mid-portion of the muscle where the fibers were allowed to freely shorten during tissue preparation (contralateral, frozen as a block). The mean CSA of each fiber type was significantly smaller in the muscles frozen at length vs. frozen as a block in both a deep (close to the bone) and a superficial (away from the bone) region of the muscle, except for the slow oxidative (SO) fibers in the superficial region. The percent difference in mean fiber CSA was smaller for the SO compared to the fast oxidative glycolytic and fast glycolytic fibers in both the deep (41, 47 and 56%, respectively) and superficial (20, 36 and 48%, respectively) regions. In addition, the differences in the mean CSAs of the fast fibers between the two freezing procedures were approximately 10% larger in the deep compared to the superficial region. The fiber-type differential responses may be related, at least in part, to the architectural features of the fibers. These data indicate that the freezing procedures used to prepare the muscle tissue are an important consideration when determining the CSA of individual skeletal muscle fibers and consequently the specific tension, tension per unit CSA, of the muscle units.