Positive influence of simvastatin used as adjuvant agent for cavity lining
Positive influence of simvastatin used as adjuvant agent for cavity lining
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DOI：
10.1007/s00784-018-2757-7
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发表时间：
2019-09-01
影响因子：
3.4
通讯作者：
de Souza Costa, Carlos Alberto
中科院分区：
文献类型：
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作者：
Leite, Maria Luisa;Soares, Diana Gabriela;de Souza Costa, Carlos Alberto
目的评价在应用玻璃离子水门汀（GIC）前应用辛伐他汀（SV）治疗深层牙本质的生物学、抗菌和力学效果。材料和方法将牙本质盘调整为人工牙髓腔，并将SV（2.5或1.0 mg/mL）涂抹在咬合表面，无论是否预先使用EDTA （+/- EDTA）。将提取液（培养基+分散在牙本质中的SV）用于培养成牙本质样MDPC-23细胞。测定细胞活力、碱性磷酸酶（ALP）活性和矿化结节（MN）沉积。未处理的椎间盘作为对照。研究了SV（2.5或1.0 mg/mL）对变形链球菌和嗜酸乳杆菌的抑菌活性，以及SV 2.5 mg/mL （+/- EDTA）存在时GIC与牙本质的结合强度。数据采用方差分析/Tukey检验（alpha = 5%）进行分析。结果与对照组相比，EDTA + SV 2.5 mg/mL显著提高了ALP活性和MN沉积，但对细胞活力无显著影响（p < 0.05）。EDTA + SV 2.5 mg/mL + GIC对ALP和MN值影响最大（p < 0.05）。SV具有较强的抗菌活性，与单独处理SV相比，SV处理后的EDTA牙本质调节提高了牙本质的结合强度值（p < 0.05）。结论SV具有抗菌活性，并能在牙本质内扩散，刺激成牙本质样牙髓细胞。
Objectives To assess the biological, antimicrobial, and mechanical effects of the treatment of deep dentin with simvastatin (SV) before application of a glass-ionomer cement (GIC). Materials and methods Dentin discs were adapted to artificial pulp chambers and SV (2.5 or 1.0 mg/mL) was applied to the occlusal surface, either previously conditioned or not with EDTA (+/- EDTA). The extracts (culture medium + SV that diffused through dentin) was obtained and then applied to cultured odontoblast-like MDPC-23 cells. Cell viability, alkaline phosphatase (ALP) activity, and mineralization nodule (MN) deposition were evaluated. Untreated discs were used as control. The antibacterial activity of SV (2.5 or 1.0 mg/mL) against Streptococcus mutans and Lactobacillus acidophilus, as well as the bond strength of GIC to dentin in the presence of SV 2.5 mg/mL (+/- EDTA) were also assessed. The data were analyzed by ANOVA/Tukey tests (alpha = 5%). Results EDTA + SV 2.5 mg/mL significantly enhanced the ALP activity and MN deposition in comparison with the control, without changing in the cell viability (p < 0.05). The association EDTA + SV 2.5 mg/mL + GIC determined the highest ALP and MN values (p < 0.05). SV presented intense antimicrobial activity, and the EDTA dentin conditioning followed by SV application increased bond strength values compared with SV treatment alone (p < 0.05). Conclusion SV presents antimicrobial activity and diffuses across conditioned dentin to biostimulate odontoblast-like pulp cells.