Epithelial Differentiation of Human Adipose-Derived Stem Cells
Epithelial Differentiation of Human Adipose-Derived Stem Cells
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DOI：
10.1007/978-1-61737-960-4_21
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Geiger, Helmut
中科院分区：
文献类型：
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作者：
Baer, Patrick C.;Brzoska, Martin;Geiger, Helmut
脂肪干细胞 (ASC) 具有分化为中胚层、内胚层和外胚层来源细胞的多功能分化潜力，使这些细胞处于细胞疗法和细胞移植的前沿。 ASC 的上皮分化可以通过直接细胞-细胞或细胞-基质接触、通过视黄酸等化学因子或通过条件培养基中包含的细胞因子、白细胞介素或生长因子等分泌细胞因子启动。本方案描述了诱导 ASC 从人类脂肪组织体外分化为上皮谱系的方法，并描述了用于验证这种诱导分化的方法。我们提出了两种基于视黄酸或培养上皮细胞条件培养基的分化方案。
The versatile differentiation potential of adipose-derived stem cells (ASC) into cells of mesodermal, entodermal, and ectodermal origin places these cells at the forefront of cell-based therapies and cell transplantation. Epithelial differentiation of ASC may either be initiated by direct cell-cell or cell-matrix contacts, by chemical factors like retinoic acid, or via secreted cellular factors like cytokines, interleukins, or growth factors included in conditioned media.This protocol describes methods to induce the in vitro differentiation of ASC from human adipose tissue into the epithelial lineage, and describes the methods used to verify this induced differentiation. We present two differentiation protocols based on either retinoic acid or conditioned medium of cultured epithelial cells.