Stimulation of sexual reproduction in Phytophthora cactorum and P-parasitica by fatty acids and related compounds
Stimulation of sexual reproduction in Phytophthora cactorum and P-parasitica by fatty acids and related compounds
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DOI：
10.1017/s0953756297004127
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发表时间：
1997-09-01
影响因子：
--
通讯作者：
Ko, WH
中科院分区：
文献类型：
--
作者：
Jee, HJ;Ko, WH
经薄层层析(TIE)纯化后，几种脂肪在致病疫霉中添加促进卵孢子的形成，而在寄生疫霉中不能。棕榈油酸是最有效的脂肪酸，也是唯一能促进寄生孢子形成的脂肪酸。用Florisil柱层析纯化棕榈油酸的活性比用TiE纯化的活性提高了一倍。在含有棕榈油酸的基础培养基中加入0.01~100 ng的胆固醇时，添加的胆固醇不会显着改变恶臭假单胞菌的卵孢子产量。TLC处理的脂肪烃或烃的衍生物对卵孢子的形成有轻微的促进作用，而香叶醇和角鲨烯对卵孢子的形成有强烈的促进作用。维生素A和维生素A酯均能促进恶臭疫霉和寄生疫霉的卵孢子形成。TIE处理还显著提高了胆固醇和β-谷甾醇对恶臭假单胞菌卵孢子形成的影响。两种化合物对寄生疫霉的卵孢子形成均无促进作用。
After purification by thin-layer chromatography (tie) several fatty adds stimulated oospore formation in Phytophthora cactorum but not in Phytophthora parasitica. Palmitoleic acid was the most effective and was the only fatty acid tested that promoted oospore formation in P. parasitica. Purification of palmitoleic acid by Florisil column chromatography resulted in a two-fold increase in activity over that purified by tie. When 0.01 to 100 ng of cholesterol was added to basal medium containing palmitoleic acid the added cholesterol did not significantly change oospore production by P. cactorum. Oospore formation in P, cactorum was slightly stimulated by tlc-treated aliphatic hydrocarbons, or derivatives of hydrocarbons, but was strongly stimulated by geraniol and squalene. Vitamin A and vitamin A esters stimulated oospore formation in both P. cactorum and P, parasitica. tie treatment also significantly increased the activity of cholesterol and beta-sitosterol on oospore formation by P. cactorum. Both compounds did not stimulate oospore formation by P, parasitica even after tie treatment.