Antifungal activity of DNA-lipid complexes and DNA-lipid films against candida species
Antifungal activity of DNA-lipid complexes and DNA-lipid films against candida species
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DOI：
10.1002/jbm.a.30394
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发表时间：
2006-01-01
影响因子：
4.9
通讯作者：
Okahata, Y
中科院分区：
文献类型：
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作者：
Inoue, Y;Fukushima, T;Okahata, Y
在这项研究中，两亲性脂质，DNA-脂质复合物，和DNA-脂质膜的制备，并对念珠菌的抗真菌活性进行了研究。在对甲苯磺酸存在下，甘氨酸或L-丙氨酸与正烷基醇反应合成了两亲性脂质。DNA-脂质复合物是由DNA和两亲性脂质简单混合而成，不溶于水。通过从氯仿/乙醇溶液中浇铸DNA脂质复合物来制备自立的水不溶性DNA脂质膜。脂质和DNA-脂质复合物对念珠菌的抗真菌活性通过最低抑菌浓度（MIC）进行了评价; DNA-脂质膜的抗真菌活性通过纸片扩散法进行了评价。7种脂质、DNA-脂质复合物和DNA-脂质膜均具有抗真菌活性，甘氨酸和L-丙氨酸衍生物的抗真菌活性无差异。脂质，DNA-脂质复合物，和DNA-脂质膜，其中具有较短的烷基链长度的脂质，对所有念珠菌的抗真菌活性。然而，对念珠菌属的抗真菌活性的效果下降，增加脂肪中的烷基链长度。在这项研究中，人们发现，脂质，DNA-脂质复合物，并与癸基或十二烷基基团的膜表现出更有利的抗真菌活性。(c)2005 Wiley Periodicals，Inc.
In this study amphiphilic lipids, DNA-lipid complexes, and DNA-lipid films were prepared, and their antifungal activity against Candida species was examined. The amphiphilic lipids were synthesized from a reaction of glycine or L-alanine with n-alkyl alcohol in the presence of p-toluene sulfonic acid. DNA-lipid complexes, which were prepared by the simple mixing of DNA and amphiphilic lipids, were insoluble in water. Self-standing, water-insoluble DNA-lipid films were prepared by casting the DNAlipid complexes from a chloroform /ethanol solution. The antifungal activities of the lipids and DNA-lipid complexes against the Candida species were evaluated by minimum inhibitory concentrations (MICs); those of DNA-lipid films were evaluated by the disk diffusion method. The seven kinds of lipids, DNA-lipid complexes, and DNA-lipid films showed antifungal activity, and no differences were seen in the antifungal activities between glycine and L-alanine derivatives. The lipids, DNA-lipid complexes, and DNA-lipid films, which have shorter alkyl chain length in lipids, showed antifungal activity against all Candida species. However, the effect of antifungal activity against Candida species decreased with increased alkyl chain length in lipids. In this study, it was found that lipids, DNA-lipid complexes, and films with a decyl or dodecyl group exhibit more favorable antifungal activity. (c) 2005 Wiley Periodicals, Inc.