Selection of monoclonal antibody to hCG which localizes in human choriocarcinoma growing in the Syrian hamster cheek pouch.
Selection of monoclonal antibody to hCG which localizes in human choriocarcinoma growing in the Syrian hamster cheek pouch.
复制标题
选择针对生长在叙利亚仓鼠颊囊中的人绒毛膜癌的 hCG 单克隆抗体。
DOI：
10.1089/hyb.1984.3.41
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Beierwaltes,WH
中科院分区：
文献类型：
--
作者：
Khazaeli,MB;Brown,LE;Kabza,GA;Sargent,EW;Beierwaltes,WH
Six different monoclonal antibodies (MAbs) to human chorionic gonadotropin (hCG) were evaluated for radioimmunodetection in Golden Syrian Hamsters bearing a human choriocarcinoma in the cheek pouch (188-516 mIU of hCG/g). The affinity of three antibodies when determined were high and five of the antibodies were of IgG1 subclass. After i.v. injection of Staphylococcus Protein A (SPA) affinity purified radioiodinated antibodies, tissue distribution studies demonstrated that antibody 061 had higher target-to-nontarget ratios than the other five antibodies. The mean tumor:muscle ratio of131I-MAb 061 was 27.5 in four animals, where the mean tumor:muscle ratio of four animals injected with131I-labeled control monoclonal IgG1Kappa from MOPC-21 murine myeloma (MOPC-21) was only 2.2. Sharp images of the cheek pouch tumor were obtained at five days following a tracer dose of131I-061 MAb, but not with the same dose of a131I-MOPC-21.
