Trends and advances in food analysis by real-time polymerase chain reaction.
Trends and advances in food analysis by real-time polymerase chain reaction.
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DOI：
10.1007/s13197-016-2205-0
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
Ahmed MU
中科院分区：
文献类型：
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作者：
Salihah NT;Hossain MM;Lubis H;Ahmed MU
由于粮食数量、多样性和流动性的迅速变化，确保粮食安全和质量的分析现在更加重要。必须确定食品污染，以维护健康和法律，以及道德和文化问题。实时聚合酶链反应（RT-PCR）是一种快速、廉价的定量方法，可用于检测样品中目标DNA片段的存在，有助于确定生物污染物对食品的意外和故意掺假。本文综述了RT-PCR在食品分析中的理论、技术和应用方面的最新进展。RT-PCR解决了传统食品分析在灵敏度、分析物范围、多路复用能力、成本、时间和床旁应用方面的局限性。一系列目标，包括用作食品成分的植物或动物物种，食源性细菌或病毒，转基因生物和过敏原，即使在高度加工的食品中，即使在非常低的浓度下也可以通过RT-PCR识别。微流控RT-PCR消除了单独的样品处理步骤，为即时分析创造了机会。我们还介绍了使用RT-PCR进行食品分析所面临的挑战，例如需要进一步改进样品处理。本文的在线版本（doi：10.1007/s13197-016-2205-0）包含补充材料，可供授权用户使用。
Analyses to ensure food safety and quality are more relevant now because of rapid changes in the quantity, diversity and mobility of food. Food-contamination must be determined to maintain health and up-hold laws, as well as for ethical and cultural concerns. Real-time polymerase chain reaction (RT-PCR), a rapid and inexpensive quantitative method to detect the presence of targeted DNA-segments in samples, helps in determining both accidental and intentional adulterations of foods by biological contaminants. This review presents recent developments in theory, techniques, and applications of RT-PCR in food analyses, RT-PCR addresses the limitations of traditional food analyses in terms of sensitivity, range of analytes, multiplexing ability, cost, time, and point-of-care applications. A range of targets, including species of plants or animals which are used as food ingredients, food-borne bacteria or viruses, genetically modified organisms, and allergens, even in highly processed foods can be identified by RT-PCR, even at very low concentrations. Microfluidic RT-PCR eliminates the separate sample-processing step to create opportunities for point-of-care analyses. We also cover the challenges related to using RT-PCR for food analyses, such as the need to further improve sample handling. The online version of this article (doi:10.1007/s13197-016-2205-0) contains supplementary material, which is available to authorized users.