Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications.
Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications.
复制标题
流感嗜血杆菌疾病分离株的外膜蛋白组成：致病性和流行病学意义。
DOI：
10.1128/iai.30.3.709-717.1980
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发表时间：
1980
影响因子：
3.1
通讯作者：
Smith,DH
中科院分区：
文献类型：
--
作者：
Loeb,MR;Smith,DH
本文用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法测定了50株流感嗜血杆菌病株的外膜蛋白组成。所有菌株，包括28个菌株，血清型a、c、d、e和f各一个菌株，和17个不可分型菌株，具有革兰氏阴性菌典型的外膜蛋白组成，即，这些膜含有两到三十种蛋白质，其中四到六种蛋白质占其蛋白质含量的大部分。这些主要的外膜蛋白从菌株的流动性的变化是常见的，但不是普遍的，所观察到的模式提供了有用的数据和新的见解typebdisease的流行病学。基本发现可以总结如下：（i）所有50个菌株都具有三种蛋白质（一种次要蛋白质和两种主要蛋白质），每种蛋白质具有相同的迁移率。其他蛋白质，无论是主要的还是次要的，在流动性上各不相同。(ii)所有的B型菌株都有一个第四（主要）蛋白质的相同的流动性。(iii)根据6种主要外膜蛋白的迁移率，将28个型菌株分为8个亚型。在所有其他被检测的菌株中，无论是可分型的还是不可分型的，只有该型菌株属于这些亚型中的一种。(iv)不典型的菌株表现出相当大的变化，其主要外膜蛋白的迁移率。在这17种菌株中，13种菌株具有包裹菌株中不存在的额外主要外膜蛋白。(v)单个菌株的外膜蛋白组成在固体培养基上经过多次传代后保持不变，但随生长阶段而变化。(vi)从9例脑膜炎流行期间获得的分离株的外膜蛋白组成不同，表明一个单一的菌株是不负责的流行。至少有五种不同的菌株造成了这九例病例。(vii)在B型菌株和该菌株的一个产荚膜缺陷突变株中观察到相同的外膜蛋白组成，表明荚膜合成水平与外膜蛋白组成没有明显关系;在从急性脑膜炎患者的不同解剖部位分离的B型菌株中，表明与菌血症相关的菌株与从脑脊液中分离的菌株相同;在从大约同时感染脑膜炎的兄弟姐妹中分离的B型菌株中，表明感染了相同的菌株;从同一患者的初次和重复感染中分离出的B型菌株，表明再感染是由于同一菌株。
The outer membrane protein composition of 50 disease isolates ofHaemophilus influenzaehas been determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All strains, including 28 strains of serotypeb, one strain each of serotypesa, c, d, e, andf, and 17 untypable strains, had an outer membrane protein composition typical of gram-negative bacteria, i.e., these membranes contained two to three dozen proteins with four to six proteins accounting for most of their protein content. Variation in the mobility of these major outer membrane proteins from strain to strain was common but not universal; the observed patterns provided useful data and new insight into the epidemiology of typebdisease. The basic findings can be summarized as follows: (i) All 50 strains possessed three proteins (one minor and two major) each having identical mobilities. The other proteins, both major and minor, varied in mobility. (ii) All typebstrains possessed a fourth (major) protein of identical mobility. (iii) The 28 typebstrains, on the basis of the mobility of the six major outer membrane proteins, could be divided into eight subtypes. Of all the other strains examined, both typable and untypable, only the serotypeastrain belonged to one of these subtypes. (iv) The untypable strains showed considerable variation in the mobilities of their major outer membrane proteins. Of these 17 strains, 13 had an additional major outer membrane protein not present in encapsulated strains. (v) The outer membrane protein composition of a single strain remained unchanged after many passages on solid media, but varied with the growth phase. (vi) The outer membrane protein composition of isolates obtained from nine patients during an epidemic of typebmeningitis varied, indicating that a single strain was not responsible for the epidemic. At least five different strains were responsible for these nine cases. (vii) Identical outer membrane protein compositions were observed in the following: in a typebstrain and a mutant of this strain deficient in capsule production, indicating that the level of capsule synthesis is not obviously related to outer membrane protein composition; in typebstrains isolated from different anatomic sites of patients acutely ill with meningitis, indicating that the strain associated with bacteremia is the same as that isolated from the cerebrospinal fluid; in typebstrains isolated from siblings who contracted meningitis at about the same time, indicating infection with the same strain; and in typebstrains isolated from the initial and repeat infection of a single patient, suggesting that reinfection was due to the same strain.