CCR4+ memory CD4+ T lymphocytes are increased in peripheral blood and lesional skin from patients with atopic dermatitis
CCR4+ memory CD4+ T lymphocytes are increased in peripheral blood and lesional skin from patients with atopic dermatitis
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DOI：
10.1067/mai.2001.112601
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发表时间：
2001-02-01
影响因子：
14.2
通讯作者：
Sato, S
中科院分区：
文献类型：
--
作者：
Nakatani, T;Kaburagi, Y;Sato, S
背景资料：目的：探讨CCR 4、CXCR 3表达与T（H）2、T（H）1细胞的关系以及CCR 4、CXCR 3表达与特应性皮炎（AD）患者炎症反应的关系。流式细胞术检测血T细胞中细胞因子产生和趋化因子受体表达。结果：CCR 4（+）和CXCR 3（+）CD 4（+）T细胞主要分别产生IL-4和IFN-γ。尽管AD患者（n = 29）和健康对照受试者（n = 19）的CD 4（+）CD 45 RO（+）T细胞中CXCR 3（+）细胞的频率相似，但重度AD患者（n = 14）的CXCR 3（+）细胞频率降低。相反，CCR 4（+）细胞的频率和CCR 4/CXCR 3比值在AD患者（n = 22）中高于健康对照组（n = 16），并与AD的疾病严重程度相关。CCR 4（+）细胞频率与嗜酸性粒细胞数和血清IgE水平呈正相关，而CXCR 3（+）细胞频率与嗜酸性粒细胞数呈负相关。CCR 4（+）或CXCR 3（+）细胞的频率在银屑病患者（n = 6）和健康对照受试者中相似。免疫组化结果显示，AD患者慢性皮损中CCR 4（+）细胞在CD 4（+）T细胞中的比例高于银屑病患者（n = 4）。我们的数据表明CCR 4表达与T（H）2细胞相关，CCR 4（+）细胞在AD患者血液中占优势，CCR 4在T（H）2细胞从血液向AD皮损迁移中起重要作用。
Background: Recent studies have reported that T(H)1 and T(H)2 cells express CXCR3 and CCR4, respectively.Objective: Our goal was to assess the association of CCR4 and CXCR3 expression with T(H)2 and T(H)1 cells and association of CCR4 and CXCR3 expression with inflammation in patients with atopic dermatitis (AD).Methods: Intracellular cytokine production and chemokine receptor expression in blood T cells were examined by flow cytometry. Immunohistochemical expression of chemokine receptors was also investigated in chronically lesional skin.Results: CCR4(+) and CXCR3(+) CD4(+) T cells predominantly produced IL-4 and IFN-gamma, respectively. Although the frequency of CXCR3(+) cells among CD4(+) CD45RO(+) T cells was similar for patients with AD (n = 29) and healthy control subjects (n = 19), patients with severe AD (n = 14) had a reduced frequency of CXCR3(+) cells. In contrast, the frequency of CCR4(+) cells and the CCR4/CXCR3 ratio were higher in patients with AD (n = 22) than healthy control subjects (n = 16) and correlated with disease severity of AD. The frequency of CCR4(+) cells correlated positively with eosinophil numbers and serum IgE levels, whereas the frequency of CXCR3(+) cells correlated inversely with eosinophil numbers. The frequency of CCR4(+) or CXCR3(+) cells was similar in patients with psoriasis (n = 6) and healthy control subjects. Immunohistochemical analysis showed that the frequency of CCR4(+) cells among CD4(+) T cells in chronically lesional skin of patients with AD (n = 9) was higher than that of patients with psoriasis (n = 4).Conclusion: Our data suggest the association of CCR4 expression with T(H)2 cells, the predominance of CCR4(+) cells in blood from patients with AD, and an important role of CCR4 in the migration of T(H)2 cells from blood into AD lesional skin.