Memory Follicular Helper Invariant NKT Cells Recognize Lipid Antigens on Memory B Cells and Elicit Antibody Recall Responses
Memory Follicular Helper Invariant NKT Cells Recognize Lipid Antigens on Memory B Cells and Elicit Antibody Recall Responses
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记忆滤泡辅助恒定 NKT 细胞识别记忆 B 细胞上的脂质抗原并引发抗体回忆反应
DOI：
10.4049/jimmunol.1701026
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发表时间：
2018-05-01
影响因子：
4.4
通讯作者：
Bai, Li
中科院分区：
文献类型：
--
作者：
Chen, Zuolong;Zhu, Shasha;Bai, Li
已显示不变NKT（iNKT）细胞以同源或非同源方式帮助B细胞;然而，同源iNKT细胞是否帮助诱导B细胞记忆应答仍然存在争议，并且潜在机制仍不清楚。在这项研究中，我们证明，在缺乏滤泡辅助T细胞的情况下，同源iNKT细胞帮助可以促进小鼠的B细胞记忆反应，这依赖于记忆滤泡辅助iNKT（iNKTFH）细胞的形成及其与记忆B细胞在回忆反应中的相互作用。记忆性iNKTFH细胞的产生需要树突状细胞而不是B细胞的脂质Ag呈递。在再激发后，记忆性iNKTFH细胞识别由记忆性B细胞呈递的脂质Ag，其召回iNKTFH效应细胞并引发B细胞记忆应答。然而，LPS，促进自身脂质的合成，未能引起回忆反应的情况下，外源性脂质抗原。
Invariant NKT (iNKT) cells have been shown to help B cells in a cognate or noncognate manner; however, whether cognate iNKT cell help induces B cell memory responses remains controversial, and the underlying mechanisms are still unclear. In this study, we demonstrated that, in the absence of follicular helper T cells, cognate iNKT cell help could promote B cell memory responses in mice that were dependent on the formation of memory follicular helper iNKT (iNKTFH) cells and their interactions with memory B cells in recall responses. Generation of memory iNKTFH cells required lipid Ag presentation by dendritic cells but not by B cells. Upon rechallenge, memory iNKTFH cells recognized lipid Ags presented by memory B cells, which recalled iNKTFH effector cells and elicited B cell memory responses. However, LPS, which promoted the synthesis of self-lipids, failed to elicit recall responses in the absence of exogenous lipid Ags.