CLONING THE MESSENGER-RNA ENCODING 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE, THE KEY ENZYME FOR ETHYLENE BIOSYNTHESIS IN PLANTS
CLONING THE MESSENGER-RNA ENCODING 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE, THE KEY ENZYME FOR ETHYLENE BIOSYNTHESIS IN PLANTS
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DOI：
10.1073/pnas.86.17.6621
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发表时间：
1989-09-01
影响因子：
11.1
通讯作者：
THEOLOGIS, A
中科院分区：
文献类型：
--
作者：
SATO, T;THEOLOGIS, A
乙烯是植物激素，控制植物生长和发育的几个特征。其合成中的限速步骤是由S-腺苷甲硫氨酸（ACC合酶催化的S-腺苷甲硫氨酸）形成乙烯前体1-氨基环丙烷-1-羧酸（ACC）。我们从西葫芦果实中分离到一个编码ACC合成酶的互补DNA序列。克隆的生物活性通过克隆的序列在大肠杆菌和酵母中指导ACC合酶活性的能力来证实。以ACC cDNA为探针的体内研究表明，ACC合酶基因受多种诱导物的诱导，包括创伤、Li+离子和植物激素生长素。
Ethylene is the plant hormone that controls several features of plant growth and development. The rate-limiting step in its synthesis is the formation of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) from S-adenosylmethionine (AdoMet), catalyzed by ACC synthase. We have isolated a complementary DNA sequence encoding ACC synthase from zucchini (Cucurbita) fruits. The biological activity of the clone was confirmed by the ability of the cloned sequence to direct ACC synthase activity in Escherichia coli and yeast. In vivo studies using the ACC cDNA as probe showed that the ACC synthase gene is induced by diverse group of inducers, including wounding, Li+ ions, and the plant hormone auxin.