A serine protease-encoding gene that marks activated cytotoxic T cells in vivo and in vitro.
A serine protease-encoding gene that marks activated cytotoxic T cells in vivo and in vitro.
复制标题
丝氨酸蛋白酶编码基因，在体内和体外标记激活的细胞毒性 T 细胞。
DOI：
10.1007/978-3-642-73911-8_7
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发表时间：
1989
影响因子：
--
通讯作者：
Weissman,IL
中科院分区：
文献类型：
--
作者：
Hershberger,RJ;Mueller,C;Gershenfeld,HK;Weissman,IL
活化的细胞毒性T淋巴细胞（ctl）被认为是病毒感染或转化的宿主细胞裂解的效应器，移植物排斥反应的介质，以及许多自身免疫性疾病的潜在罪魁祸首。不幸的是，目前还没有标志物可以在体内特异性识别这些细胞，因此很难明确证明ctl是这些免疫功能中的效应细胞。成熟T细胞通常根据其表面表型分为亚群：表达CD8抗原的T细胞被认为是潜在的细胞毒性细胞，表达CD4抗原的T细胞被认为是潜在的辅助细胞。然而，CD4+CD8-CTLs （Goldinget al. 1985）和CD4- cd8 +辅助细胞（SwainandPanfili1979）在体外和体内移植排斥反应中的鉴定（Rosenberget al. 1987）限制了这种分裂的有效性。使用表型来暗示功能的一个更严重的问题是，这些标记在活性T细胞和静止T细胞上都表达。因此，它们不能用于区分参与局部免疫反应的细胞和非特异性存在于该部位的细胞。为了更准确地鉴定细胞毒性淋巴细胞，我们使用分子遗传学方法分离这些细胞表达的基因。
Activated cytotoxic T lymphocytes (CTLs) are presumed to be the effectors in the lysis of virally infected or transformed host cells, the mediators of graft rejection, and the potential culprits in a number of autoimmune diseases. Unfortunately, there is no marker available that specifically identifies these cells in vivo, making it difficult to prove unequivocally that CTLs are the effector cells in these immune functions. Mature T cells are usually divided into subsets on the basis of their surface phenotype: Those that express the CD8 antigen are assumed to be potential cytotoxic cells, and those that express the CD4 antigen, potential helper cells. However, the identification of CD4+CD8-CTLs (Goldinget al. 1985) and CD4-CD8+helper cells (SwainandPanfili1979) both in vitro and in graft rejection in vivo (Rosenberget al. 1987) limits the usefulness of this division. A more serious problem with using phenotype to imply function is that these markers are expressed on both active and resting T cells. Thus, they cannot be used to distinguish between the cells participating in a localized immune response and those nonspecifically present at the site. To identify cytotoxic lymphocytes more accurately, we used a molecular genetic approach to isolate genes expressed by these cells.