TRANSIENT ACCUMULATION OF NITRITE REDUCTASE MESSENGER-RNA IN MAIZE FOLLOWING THE ADDITION OF NITRATE
TRANSIENT ACCUMULATION OF NITRITE REDUCTASE MESSENGER-RNA IN MAIZE FOLLOWING THE ADDITION OF NITRATE
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DOI：
10.1104/pp.90.3.1214
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发表时间：
1989-07-01
期刊：
影响因子：
7.4
通讯作者：
ROTHSTEIN, S
中科院分区：
文献类型：
--
作者：
KRAMER, V;LAHNERS, K;ROTHSTEIN, S
亚硝酸盐还原酶（NiR）的基因编码的表达在添加硝酸盐后被诱导。我们分析了这种诱导过程中水培生长的玉米（玉米L。）幼苗的培养基中的硝酸盐水平可以很容易地操纵。有一个快速的诱导NiR mRNA在添加硝酸盐，增加首先在根中，然后在叶中。反应的速度取决于硝酸盐浓度和生长培养基。然而，表达的一般模式是相同的; mRNA水平增加，达到最大值，然后降低，尽管培养基中的硝酸盐浓度保持不变。这种mRNA水平的下降可以相当迅速，特别是在根组织中。如果硝酸盐作为脉冲给予，mRNA水平下降得更快。很明显，NiR mRNA是短暂的，在根部的半衰期不到30分钟。另一方面，NiR蛋白水平在mRNA增加后逐渐增加，并且在添加硝酸盐后至少24小时保持在高水平。
Expression of the gene coding for nitrite reductase (NiR) is induced upon the addition of nitrate. We have analyzed this induction process in hydroponically grown maize (Zea mays L.) seedlings where the level of nitrate in the medium can be easily manipulated. There is a rapid induction of NiR mRNA upon addition of nitrate, increasing first in the roots and then in the leaves. The rapidity of the response depends on the nitrate concentration and the growth medium. However, the general pattern of expression is the same; the mRNA level increases, reaches a maximum, and then decreases, despite the fact that the nitrate concentration in the medium remains constant. This decline in mRNA level can be quite rapid, particularly in root tissue. If the nitrate is given as a pulse, the mRNA levels decrease even more rapidly. It is clear that the NiR mRNA is short-lived, with a half-life in the roots of less than 30 minutes. The NiR protein level, on the other hand, increases gradually somewhat after the increase in mRNA and remains at high levels at least for 24 hours after the addition of nitrate.