Expression of CD44 variant isoforms CD44v3 and CD44v6 is increased on T cells from patients with systemic lupus erythematosus and is correlated with disease activity.
Expression of CD44 variant isoforms CD44v3 and CD44v6 is increased on T cells from patients with systemic lupus erythematosus and is correlated with disease activity.
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DOI：
10.1002/art.27385
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发表时间：
2010-05
影响因子：
--
通讯作者：
Costenbader, Karen H.
中科院分区：
文献类型：
--
作者：
Crispin, Jose C.;Keenan, Brendan T.;Finnell, Michele D.;Bermas, Bonnie L.;Schur, Peter;Massarotti, Elena;Karlson, Elizabeth W.;Fitzgerald, Lisa M.;Ergin, Sukran;Kyttaris, Vasileios C.;Tsokos, George C.;Costenbader, Karen H.
本研究对系统性红斑狼疮(SLE)患者T细胞表面CD44及其异构体CD44v3和CD44v6的表达进行了定量研究，并探讨其表达水平与疾病表现的关系。收集了有关临床和人口学特征的信息，并从72名SLE患者和32名按性别、种族和年龄与患者匹配的健康对照组采集了血液样本。用流式细胞仪检测T细胞亚群CD44及其变异体CD44v3和V6的表达，并分析其表达水平与临床变量、SLE疾病活动指数(SLEDAI)和狼疮性肾炎的相关性。用Wilcoxon‘s秩和检验和条件多变量回归分析确定CD44在SLE患者和健康对照组之间的表达差异。系统性红斑狼疮患者CD_4~+、CD_8~+T细胞表面CD_(44)的表达明显高于对照组(P<0.05)(P<≤)。系统性红斑狼疮患者外周血中总T细胞、CD_4~+、CD_8~+T细胞CD44v3、CD_(44)v_6的表达也明显高于对照组(P<≤)。SLEDAI评分与总T细胞、CD4+T细胞、CD8+T细胞表面CD44v3、CD44v6的表达水平呈正相关(P<0.05)。狼疮性肾炎的发生与CD_4~+T细胞、CD_4~+T细胞、CD_4~−、CD_8~+−T细胞上CD44v6的表达有关(P<0.05)。抗双链DNA抗体阳性与T细胞CD44v6表达水平相关(P<；0.05)。提示T细胞CD44v3和CD44v6的表达水平可能是反映SLE活动性的有用生物标志物。
This study was performed to quantify the expression of CD44 and variant isoforms CD44v3 and CD44v6 on T cells from patients with systemic lupus erythematosus (SLE), and to correlate the level of expression of these molecules with disease manifestations. Information on clinical and demographic characteristics was collected and blood samples were obtained from 72 patients with SLE and 32 healthy control subjects matched to the patients by sex, race, and age. Expression of CD44 and variants CD44v3 and v6 on T cell subsets was determined by flow cytometry, and Pearson’s correlations of their expression levels with clinical variables, SLE Disease Activity Index (SLEDAI) scores, and presence of lupus nephritis were determined. Wilcoxon’s rank sum tests and conditional multivariable regression analyses were applied to identify differences in the expression of CD44 between patients with SLE and healthy controls. Expression of CD44 was higher on CD4+ and CD8+ T cells from SLE patients compared with controls (P ≤ 0.03). Expression of CD44v3 and CD44v6 was also higher on total T cells and CD4+ and CD8+ T cells from SLE patients compared with controls (P ≤ 0.03). Cell surface levels of CD44v3 on total T cells, CD4+ T cells, and CD8+ T cells as well as cell surface expression of CD44v6 on total T cells and CD4+ T cells were correlated with the SLEDAI score (P < 0.05). The presence of lupus nephritis was associated with the expression of CD44v6 on total T cells, CD4+ T cells, and CD4−CD8− T cells (P < 0.05). Positivity for anti–double-stranded DNA antibodies was associated with the expression levels of CD44v6 on T cells (P < 0.05). These results indicate that expression levels of CD44v3 and CD44v6 on T cells may represent useful biomarkers of SLE activity.
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DOI：
10.1083/jcb.128.4.673
发表时间：
1995-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Jackson DG;Bell JI;Dickinson R;Timans J;Shields J;Whittle N
通讯作者：
Whittle N
DOI：
10.1083/jcb.135.4.1139
发表时间：
1996-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sleeman J;Rudy W;Hofmann M;Moll J;Herrlich P;Ponta H
通讯作者：
Ponta H
影响因子：
4.4
作者：
Seiter, S;Schmidt, DS;Zöller, M
通讯作者：
Zöller, M
影响因子：
5.2
作者：
Klingbeil, Pamela;Marhaba, Rachid;Zoeller, Margot
通讯作者：
Zoeller, Margot
影响因子：
4.4
作者：
Li, Yansong;Harada, Tatsuhiro;Tsokos, George C.
通讯作者：
Tsokos, George C.