Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow.
Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow.
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整合素相关蛋白 (CD47/IAP) 有助于 T 细胞在流动下停滞在炎症血管内皮上。
DOI：
10.1096/fj.99-0833com
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Bernard,A
中科院分区：
文献类型：
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作者：
Ticchioni,M;Raimondi,V;Lamy,L;Wijdenes,J;Lindberg,FP;Brown,EJ;Bernard,A
整合素相关蛋白(CD47/IAP)是一种调节整合素功能的五聚体分子。我们制备了CD47缺陷的Jurkat T细胞系，以评估其在T细胞在炎性内皮上的阻断中的作用。在流动条件下，与原始细胞系相比，CD47缺陷细胞的结构性停滞显著减少，而CD47的重新表达则恢复了它们的停滞能力。此外，用CD47的胞外部分和CD7的跨膜区或几种截短形式的CD47制成的嵌合体转染细胞，表明第一个跨膜区和一个较短的细胞质环足以完成这一过程。CD47的作用是间接的，主要依赖于α4β1/VCAM1途径，阻断抗体显示。我们在内皮细胞上检测到了迄今已知的两种CD47受体：凝血酶敏感蛋白和SIRP1α。阻断实验表明，两者都参与其中。Ticchioni，M.，Raimondi，V.，Lamy，L.，Wijdenes，J.，Lindberg，F.P.，Brown，E.J.，Bernard，A。
Integrin‐associated protein (CD47/IAP) is a pentaspan molecule that regulates integrin functions. We prepared a CD47‐deficient Jurkat T cell line to assess its role in the arrest of T cells on inflammatory endothelium. Under flow conditions, constitutive arrest of CD47‐deficient cells is strongly decreased as compared to the original cell line, whereas reexpression of CD47 reestablishes their ability to stop. Moreover, cells transfected with a chimera made with the extracellular portion of CD47 and the transmembrane domain of CD7 or several truncated forms of CD47 show that the first transmembrane domain and a short cytoplasmic loop are sufficient for this process. CD47 effect is indirect and depends mainly on theα4β1/VCAM‐1 pathway, as shown by blocking antibodies. We detected on endothelium the two CD47 counter receptors known to date: thrombospondin and SIRP1α. Blocking experiments show that both are involved. Overall, CD47 participates in the constitutive arrest of T lymphocytes on inflamed vascular endothelium by up‐regulatinga4β1 integrins.—Ticchioni, M., Raimondi, V., Lamy, L., Wijdenes, J., Lindberg, F. P., Brown, E. J., Bernard, A. Integrin‐associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow.FASEB J.15, 341‐350 (2001)