Effect of transforming growth factor β2 on marrow-infused foam poly(propylene fumarate) tissue-engineered constructs for the repair of critical-size cranial defects in rabbits
Effect of transforming growth factor β2 on marrow-infused foam poly(propylene fumarate) tissue-engineered constructs for the repair of critical-size cranial defects in rabbits
复制标题
DOI：
10.1089/ten.2005.11.923
复制
发表时间：
2005-05-01
影响因子：
--
通讯作者：
Mikos, AG
中科院分区：
文献类型：
--
作者：
Dean, D;Wolfe, MS;Mikos, AG
本研究研究了4月龄兔（n = 51）在6周或12周死亡的临界尺寸（直径1.6 cm）颅骨缺损中聚马酸丙烯（PPF）与β -磷酸三钙（β - tcp）支架的骨整合。采用两种分子量的PPF制备双层支架，外层为0.5 mm的固体层，内层为2.0 mm的多孔层。多孔层注入骨髓抽液，一半动物接受0.8 μ g转化生长因子β (tgf - β(2))。外植体或组织学检查未见异物或炎症反应。对新骨形成的组织学面积和线性测量的统计分析发现，在牺牲时间较晚时，植入物接受tgf - β(2)，然后是低分子量PPF植入物。大约40%的外植体用一点“推入”试验进行了掺入强度测试。由于没有使用永久固定，种植体强度（28.37 - 129.03 N；范围，6.4 - 29.0 lb阻力）完全是由于新骨形成所致。在12周死亡的动物中，接受tgf - β(2)注入骨髓的植入物的骨骼最强。这些结果支持PPF作为成骨基质的使用，以及对临界尺寸和超临界尺寸颅骨假体植入物术前制造的未来研究。
This study investigates the osseointegration of poly(propylene fumarate) (PPF) with beta-tricalcium phosphate (beta-TCP) scaffolds in a critical- size ( diameter, 1.6 cm), cranial defect in 4-month-old rabbits (n = 51), killed at 6 or 12 weeks. Two molecular weights of PPF were used to produce bilayer scaffolds with 0.5-mm solid external and 2.0- mm porous internal layers. The porous layer was infused with bone marrow aspirate, with half the animals receiving 0.8 mu g of transforming growth factor beta(2) (TGF-beta(2)). No foreign body or inflammatory response was observed externally or on histological examination of explants. Statistical analysis of histological areal and linear measures of new bone formation found significantly more bone at the later sacrifice time, followed by implants receiving TGF-beta(2), followed by low molecular weight PPF implants. Approximately 40% of the explants were tested for incorporation strength with a one- point "push- in" test. Because no permanent fixation was used, implant strength (28.37 - 129.03 N; range, 6.4 to 29.0 lb of resistance) was due entirely to new bone formation. The strongest bone was seen in implants receiving TGF-beta(2)-infused marrow in animals killed at 12 weeks. These results support the use of PPF as an osteogenic substrate and future research into preoperative fabrication of critical size and supercritical- size cranial prosthetic implants.