Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-kappa B
Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-kappa B
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DOI：
10.1016/s0014-5793(96)01502-5
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发表时间：
1997-01-27
期刊：
影响因子：
3.5
通讯作者：
Heinrich, M
中科院分区：
文献类型：
--
作者：
Bork, PM;Schmitz, ML;Heinrich, M
研究了54种墨西哥印第安药用植物对转录因子NF-κ B激活的潜在抑制作用，通过带移实验鉴定了红毛蒿叶乙醇提取物。Mexicana、Calea zacatechichi和Polymnia maculata（均富含倍半萜内酯）作为NF-κ B抑制剂，浓度低至25 μ g/ml。低至5 μ M时，倍半萜内酯异海伦宁和小白菊完全阻止NF-κ B活化。用紫云英叶提取物处理HeLa细胞。鲁多西亚亚种这些实验鉴定了桉叶内酯和大根香叶内酯类型的倍半萜内酯作为NF-κ B的有效的非抗氧化剂抑制剂。在NF-κ B测定中有活性的所有植物还显示在抗炎活性的生理模型-HET-CAM测定中尿囊膜的毛细血管反应的发生延迟。
The potential inhibitory effect of 54 Mexican Indian medicinal plants on the activation of transcription factor NF-kappa B was studied, Band-shift experiments identified the ethanolic leaf extracts of Artemisia ludoviciana ssp. mexicana, Calea zacatechichi, and Polymnia maculata (all rich in sesquiterpene lactones) as inhibitors of NF-kappa B down to a concentration of 25 mu g/ml. The sesquiterpene lactones isohelenin and parthenolide prevented NF-kappa B activation completely as low as 5 mu M. Treatment of HeLa cells with leaf extract of A. ludoviciana ssp. mexicana, isohelenin and parthenolide prevented the induction of transcription on the IL-6 promoter, These experiments identify the eudesmanolide and germacranolide type of sesquiterpene lactones as potent non-antioxidant inhibitors of NF-kappa B, All plants active in the NF-kappa B assay also showed a delay in the onset of capillary reactions of the allantois membrane in a physiological model for anti-inflammatory activity - the HET-CAM assay.