The composition and structure of bovine peritubular dentin: Mapping by time of flight secondary ion mass spectroscopy
The composition and structure of bovine peritubular dentin: Mapping by time of flight secondary ion mass spectroscopy
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DOI：
10.1016/j.jsb.2006.02.005
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发表时间：
2006-11-01
影响因子：
3
通讯作者：
Veis, Arthur
中科院分区：
文献类型：
--
作者：
Gotliv, Bat-Ami;Robach, Joshua S.;Veis, Arthur
牙齿的牙本质层是一种复杂的矿化组织，由紧密排列的小管系统穿过。每个小管被高度矿化的组织包围，称为小管周围牙本质（PTD）。小管之间剩余的矿化胶原网络是小管间牙本质（ITD）。PTD组分的TOF-SIMS分析已用于比较PTD与ITD。PTD与ITD的不同之处不仅在于矿化程度，还在于矿物质元素和氨基酸的量和性质。PTD的有机基质由富含谷氨酸的蛋白质的独特无胶原组装体组成，其中ITD有机基质富含胶原和Asp-rich。在TOF-SINIS负离子模式下，在PTD中观察到的有机碎片离子的表观浓度远高于预期。PTD富含Ca ~（2+）、Na ~+、Mg ~（2+）和K ~+。脱蛋白后，PTD中Mg ~（2+）和K ~+的含量显著降低，而Ca ~（2+）和Na ~+仍在PTD中积累。这意味着Mg ~（2+）和K ~+主要与有机基质有关，而不是与PTD的矿物有关。(c)2006年爱思唯尔公司All rights reserved.
The dentin layer of the tooth is a complex mineralized tissue traversed by a closely packed system of tubules. Each tubule is surrounded by highly mineralized tissue referred to as peritubular dentin (PTD). The remaining mineralized collagen network between the tubules is the intertubular dentin (ITD). A TOF-SIMS analysis of the PTD constituents has been used to compare the PTD to the ITD. The PTD differs from the ITD not only in the degree of mineralization but also in the amount and nature of the mineral elements and amino acids. The organic matrix of the PTD consists of a unique collagen free assembly of proteins rich in glutamic acid, where the ITD organic matrix is collagen-rich and Asp-rich. The apparent concentration of organic fragment ions observed in the PTD in the TOF-SINIS negative ion mode was much higher than expected. The PTD was found to be rich in Ca2+, Na+, Mg2+, and K+. The amount of Mg2+ and K+ in the PTD was significantly reduced after deproteination, while Ca2+ and Na+ were still accumulated in the PTD. This implies that Mg2+ and K+ are mainly associated with the organic matrix rather than with the mineral of the PTD. (c) 2006 Elsevier Inc. All rights reserved.