The immunogenicity of the liposome-associated outer membrane proteins (OMPs) of Moraxella catarrhalis.
The immunogenicity of the liposome-associated outer membrane proteins (OMPs) of Moraxella catarrhalis.
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DOI：
10.2478/s11658-009-0035-z
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发表时间：
2010
影响因子：
8.3
通讯作者：
Gutowicz J
中科院分区：
文献类型：
--
作者：
Augustyniak D;Mleczko J;Gutowicz J
外膜蛋白（OMP）是卡他莫拉菌疫苗抗原中最具免疫原性和吸引力的，可诱导保护性免疫反应。本研究的目的是确定两种类型的 OMP 相关磷脂酰胆碱 (PC) 脂质体制剂（OMP-PC、PC-OMP）和基于两性试剂的蛋白胶束 (OMP-Z) 在增强小鼠抗 OMP 全身免疫反应方面的有效性。通过使用 ELISA 和蛋白质印迹评估免疫后小鼠抗血清中的血清抗 OMP IgG 和 IgA 反应性来评估上述制剂的免疫原性。此外，最有效的抗 OMP 反应的交叉反应性是使用人类和小鼠的异源血清确定的。蛋白脂质体和蛋白胶束均表现出高免疫原性，并且不会引起抗体滴度的任何明显的定量差异或小鼠抗血清模式的定性差异。免疫后的小鼠抗血清主要识别粘膜炎莫拉氏菌的~60-kDa OMP。还发现该蛋白质是一种高度交叉反应性抗原，与一组通过全细胞或异源粘膜炎莫拉氏菌菌株纯化的 OMP 免疫产生的混合小鼠抗血清相互作用。此外，发现从健康儿童收集的正常血清优先与 60-kDa OMP 发生反应。通过免疫印迹法分别在90%、85%和30%的异源人血清中检测到血清特异性IgG、IgA和IgM。两种 OMP 相关脂质体制剂的这种相似的免疫原性有效性可能有助于此类制剂未来在人类疫苗接种中的实际应用。此外，高度交叉反应的 60-kDa OMP 似乎是卡他莫拉氏菌的重要抗原标记，并且由于它负责诱导抗体介导的持久免疫反应，因此研究它可能会部分帮助我们了解该细菌在免疫功能正常的个体中相对较低的致病性。
The outer membrane proteins (OMPs) are the most immunogenic and attractive of the Moraxella catarrhalis vaccine antigens that may induce the protective immune response. The aim of this study was to determine the effectiveness of two types of OMP-associated phosphatidylcholine (PC) liposomal formulations (OMPs-PC, PC-OMPs) and of Zwittergent-based proteomicelles (OMPs-Z) in potentiating an anti-OMP systemic immune response in mice. The immunogenicities of the above preparations were evaluated by assessing serum anti-OMP IgG and IgA reactivity in the post-immunized mouse antisera using ELISA and Western blotting. Additionally, the cross-reactivity of the most effective anti-OMP response was determined using heterologous sera from both humans and mice. Both the proteoliposomes and the proteomicelles showed high immunogenic properties and did not elicit any distinct quantitative differences in the antibody titer or qualitative differences in the pattern of the mouse antisera. The post-immunized mouse antisera predominantly recognized a ∼60-kDa OMP of M. catarrhalis. That protein was also found to be a highly cross-reactive antigen interacting with a panel of pooled mouse antisera produced by immunization either with whole cells or the purified OMPs of heterologous M. catarrhalis strains. Furthermore, normal sera collected from healthy children were found to be preferentially reactive with the 60-kDa OMP. The serum-specific IgG, IgA and IgM were respectively detected via immunoblotting in 90%, 85% and 30% of heterologous human sera. This similar immunogenic effectiveness of both OMP-associated liposomal formulations could contribute to the practical use of such formulations in the future in human vaccination. Moreover, the highly cross-reactive 60-kDa OMP seems to be an important antigenic marker of M. catarrhalis, and, as it is responsible for the induction of an antibody-mediated and long-lasting immune response, studying it may partially aid us in understanding the relatively low degree of pathogenicity of the bacterium in immunocompetent individuals.