Overexpression of T-type calcium channels in HEK-293 cells increases intracellular calcium without affecting cellular proliferation
Overexpression of T-type calcium channels in HEK-293 cells increases intracellular calcium without affecting cellular proliferation
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DOI：
10.1016/s0014-5793(00)01832-9
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发表时间：
2000-07-28
期刊：
影响因子：
3.5
通讯作者：
Lory, P
中科院分区：
文献类型：
--
作者：
Chemin, J;Monteil, A;Lory, P
低电压激活的T型钙通道表达增加与多种细胞事件相关，包括细胞增殖和细胞周期动力学。最近克隆了三个编码T型α(1)亚单位的基因，α(1G)，α(1H)和α(11)，NON，通过在人胚胎肾(HEK-293)细胞中过表达人α(1G)和α(1H)亚基，我们可以直接评估它们参与介导细胞增殖的作用。虽然T型通道介导了细胞内钙离子浓度的升高，但溴脱氧尿嘧啶核苷掺入和流式细胞仪分析没有显著变化。这些结果表明，T型钙通道的表达不足以调节HEK-293细胞的增殖。(C)2000年欧洲生化学会联合会。爱思唯尔科学公司出版。版权所有。
Increased expression of low voltage-activated T-type Ca2+ channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(11), non allows direct assessment of their involvement in mediating cellular proliferation, By overexpressing the human alpha(1G) and alpha(1H) subunits in human embryonic kidney (HEK-293) cells, we describe here that. although T-type channels mediate increases in intracellular Ca2+ concentrations, there is no significant change in bromodeoxyuridine incorporation and flow cytometric analysis. These results demonstrate that expressions of T-type Ca2+ channels are not sufficient to modulate cellular proliferation of HEK-293 cells. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.