The hypothalamo-hypophyseal system of the white seabream Diplodus sargus:: Immunocytochemical identification of arginine-vasotocin, isotocin, melanin-concentrating hormone and corticotropin-releasing factor
The hypothalamo-hypophyseal system of the white seabream Diplodus sargus:: Immunocytochemical identification of arginine-vasotocin, isotocin, melanin-concentrating hormone and corticotropin-releasing factor
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DOI：
10.1023/a:1014912110318
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发表时间：
2001-09-01
期刊：
影响因子：
--
通讯作者：
Mancera, JM
中科院分区：
文献类型：
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作者：
Duarte, G;Segura-Noguera, MM;Mancera, JM
应用免疫细胞化学技术研究了神经分泌激素催产素、异催产素、黑色素浓集素和促垂体激素促肾上腺皮质激素释放因子在白色黑鲷（Diplodus sargus）下丘脑-垂体系统中的分布。大细胞和小细胞胞体免疫反应阳性的催产素和异催产素在视前核。血管紧张素-催产素免疫反应的胞体比异催产素免疫反应的胞体更向尾部延伸。小细胞胞体位于核的吻腹侧，大细胞胞体位于核的背尾侧。精氨酸-催产素和异催产素不能共存于同一神经元。神经垂体的所有区域都有血管缩宫素和异催产素免疫反应阳性的纤维分布，并终止于靠近促皮质和促黑素细胞的区域。黑色素浓集激素和促肾上腺皮质激素释放因子的胞体分布于结节外侧核，室周后核也有少量神经元。此外，黑色素浓集激素免疫反应的胞体检测外侧隐窝核。视前核对这些抗血清没有免疫反应性。纤维显示黑色素浓缩激素和促肾上腺皮质激素释放因子免疫反应结束接近黑素和体乳营养细胞的中间部，并接近亲皮质细胞的吻部远侧。
The distribution of the neurosecretory hormones vasotocin, isotocin and melanin-concentrating hormone and the hypophysiotropic hormone corticotropin-releasing factor was studied in the hypothalamo-hypophyseal system of the white seabream (Diplodus sargus) using immunocytochemical techniques. Magnocellular and parvocellular perikarya immunoreactive for arginine-vasotocin and isotocin were present in the nucleus preopticus. Perikarya immunoreactive for arginine-vasotocin extended more caudally with respect to isotocin-immunoreactive perikarya. Parvocellular perikarya were located at rostroventral levels and magnocellular perikarya in the dorsocaudal portion of the nucleus. Arginine-vasotocin and isotocin did not coexist in the same neuron. Fibres immunoreactive for arginine-vasotocin and isotocin innervated all areas of neurohypophysis and terminate close to corticotropic and melanotropic cells. Perikarya immunoreactive for melanin-concentrating hormone and corticotropin-releasing factor were observed in the nucleus lateralis tuberis, with a few neurons in the nucleus periventricularis posterior. In addition, melanin-concentrating hormone immunoreactive perikarya were detected in the nucleus recessus lateralis. The preoptic nucleus did not show immunoreactivity for these antisera. Fibres showing melanin-concentrating hormone and corticotropin-releasing factor immunoreactivity ended close to the melanotropic and somatolactotrophic cells of the pars intermedia, and close to the corticotrophic cells of the rostral pars distalis.