Malate synthase gene AoMls in the nematode-trapping fungus Arthrobotrys oligospora contributes to conidiation, trap formation, and pathogenicity
Malate synthase gene AoMls in the nematode-trapping fungus Arthrobotrys oligospora contributes to conidiation, trap formation, and pathogenicity
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DOI：
10.1007/s00253-013-5432-6
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发表时间：
2013-12
影响因子：
5
通讯作者：
Xinying Zhao;Yunchuan Wang;Yong Zhao;Yan Huang;Ke-Qin Zhang;Jinkui Yang
中科院分区：
文献类型：
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作者：
Xinying Zhao;Yunchuan Wang;Yong Zhao;Yan Huang;Ke-Qin Zhang;Jinkui Yang
苹果酸合酶 (Mls) 是乙醛酸循环中的关键酶，是微生物病原体毒力所必需的。在这项研究中，我们从捕获线虫的真菌Arthobotrysolispora中鉴定出了AoMls基因。该基因含有4个内含子，编码540个氨基酸的多肽。表征AoMlsinA的功能。寡孢子菌，我们通过同源重组破坏它，并通过 PCR 和 Southern blot 分析证实了 ΔAoMls 突变体。 ΔAoMls突变体在马铃薯葡萄糖琼脂（PDA）平板上的生长速度和菌落形态与野生型菌株没有明显差异。然而，基因AoMls的破坏导致分生孢子显着减少，无法利用脂肪酸和乙酸钠生长，其分生孢子在补充油酸钠的基本培养基上无法萌发。此外，ΔAoMls突变体中陷阱的形成被延迟，其仅产生包含一或两个环的不成熟陷阱。此外，ΔAoMls突变体的杀线虫活性显着降低。我们的结果表明AoMls基因在A.的分生孢子形成、陷阱形成和致病性中发挥重要作用。少孢子虫。
Malate synthase (Mls), a key enzyme in the glyoxylate cycle, is required for virulence in microbial pathogens. In this study, we identified theAoMlsgene from the nematode-trapping fungusArthobotrys oligospora. The gene contains 4 introns and encodes a polypeptide of 540 amino acids. To characterize the function ofAoMlsinA. oligospora, we disrupted it by homologous recombination, and the ΔAoMlsmutants were confirmed by PCR and Southern blot analyses. The growth rate and colony morphology of the ΔAoMlsmutants showed no obvious difference from the wild-type strains on potato dextrose agar (PDA) plate. However, the disruption of geneAoMlsled to a significant reduction in conidiation, failure to utilize fatty acids and sodium acetate for growth, and its conidia were unable to germinate on minimal medium supplemented with sodium oleate. In addition, the trap formation was retarded in the ΔAoMlsmutants, which only produced immature traps containing one or two rings. Moreover, the nematicidal activity of the ΔAoMlsmutants was significantly decreased. Our results suggest that the geneAoMlsplays an important role in conidiation, trap formation and pathogenicity ofA. oligospora.