Synaptic responses evoked by tactile stimuli in Purkinje cells in mouse cerebellar cortex Crus II in vivo.
Synaptic responses evoked by tactile stimuli in Purkinje cells in mouse cerebellar cortex Crus II in vivo.
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体内小鼠小脑皮质 Crus II 浦肯野细胞中触觉刺激引起的突触反应
DOI：
10.1371/journal.pone.0022752
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Qiu DL
中科院分区：
文献类型：
--
作者：
Chu CP;Bing YH;Liu QR;Qiu DL
背景感觉刺激通过苔藓样纤维-颗粒细胞通路在小脑浦肯野细胞(PC)中激发反应。然而，触觉刺激在小脑PC中诱发的突触反应的特性尚不清楚。本研究研究了乌拉坦麻醉小鼠同侧须垫上喷气刺激对PC突触反应的影响。方法和主要结果采用体细胞或树突状膜片钳记录和药理学方法，在48只乌拉坦麻醉的成年(6~8周龄)HA/ICR小鼠上记录到33个细胞。对同侧须垫的触觉刺激是通过一根12号不锈钢管与加压注射系统相连的空气喷雾提供的。在电流钳条件下(I = 0)，喷气刺激可在PC体细胞内诱发出强抑制性突触后电位(IPSP)。应用GABAA受体特异性拮抗剂SR95531可阻断IPSP，并显示刺激诱发的简单棘波放电。在电压钳条件下，触觉刺激在PC的胞体和树突中诱发了一系列瞬间的内向电流，然后是强烈的外向电流。应用SR95531阻断外向电流，并显示躯体兴奋性突触后电流(EPSCs)和PC树突内平行纤维EPSCs的时间总和。我们还证明了PC对喷气刺激的开始和抵消都有反应。结论在乌拉坦麻醉小鼠，触觉刺激可诱导PCs树突的非同步平行纤维兴奋性传入，但不能引起PCs强烈的EPSCs和棘波放电，但可迅速激活由GABAA受体介导的PCs胞体和树突的抑制性突触后电流。
Background Sensory stimuli evoke responses in cerebellar Purkinje cells (PCs) via the mossy fiber-granule cell pathway. However, the properties of synaptic responses evoked by tactile stimulation in cerebellar PCs are unknown. The present study investigated the synaptic responses of PCs in response to an air-puff stimulation on the ipsilateral whisker pad in urethane-anesthetized mice. Methods and Main Results Thirty-three PCs were recorded from 48 urethane-anesthetized adult (6–8-week-old) HA/ICR mice by somatic or dendritic patch-clamp recording and pharmacological methods. Tactile stimulation to the ipsilateral whisker pad was delivered by an air-puff through a 12-gauge stainless steel tube connected with a pressurized injection system. Under current-clamp conditions (I = 0), the air-puff stimulation evoked strong inhibitory postsynaptic potentials (IPSPs) in the somata of PCs. Application of SR95531, a specific GABAA receptor antagonist, blocked IPSPs and revealed stimulation-evoked simple spike firing. Under voltage-clamp conditions, tactile stimulation evoked a sequence of transient inward currents followed by strong outward currents in the somata and dendrites in PCs. Application of SR95531 blocked outward currents and revealed excitatory postsynaptic currents (EPSCs) in somata and a temporal summation of parallel fiber EPSCs in PC dendrites. We also demonstrated that PCs respond to both the onset and offset of the air-puff stimulation. Conclusions These findings indicated that tactile stimulation induced asynchronous parallel fiber excitatory inputs onto the dendrites of PCs, and failed to evoke strong EPSCs and spike firing in PCs, but induced the rapid activation of strong GABAA receptor-mediated inhibitory postsynaptic currents in the somata and dendrites of PCs in the cerebellar cortex Crus II in urethane-anesthetized mice.
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影响因子：
2.5
作者：
BOWER, JM;WOOLSTON, DC
通讯作者：
WOOLSTON, DC
影响因子：
5.5
作者：
Dieudonné, S
通讯作者：
Dieudonné, S
影响因子：
5.5
作者：
Holtzman, Tahl;Rajapaksa, Thimali;Edgley, Steve A.
通讯作者：
Edgley, Steve A.
影响因子：
5.5
作者：
LLANO, I;GERSCHENFELD, HM
通讯作者：
GERSCHENFELD, HM
影响因子：
5.3
作者：
Bower, James M.
通讯作者：
Bower, James M.