Chicken embryos as a potential new model for early onset type I diabetes.
Chicken embryos as a potential new model for early onset type I diabetes.
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DOI：
10.1155/2014/354094
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发表时间：
2014
影响因子：
4.3
通讯作者：
Ko GY
中科院分区：
文献类型：
--
作者：
Shi L;Ko ML;Huang CC;Park SY;Hong MP;Wu C;Ko GY
糖尿病视网膜病变（DR）是导致美国工作人群失明的主要原因。本研究旨在建立一种新的糖尿病动物模型，以解决糖尿病前期和早期患者色觉扭曲和锥体通路反应改变的问题。在胚胎第11天，分别给鸡胚胎注射链脲佐菌素（STZ）、高浓度葡萄糖（高葡萄糖）或对照物。在E18时，STZ和高糖诱导的糖尿病鸡胚均发生不同程度的白内障。链脲佐菌素糖尿病鸡胚胎的血液胰岛素、葡萄糖转运蛋白4 （Glut4）和磷酸化蛋白激酶B （pAKT）水平降低。在注射stz的E20胚胎中，a波和b波的ERG振幅均显著降低，a波隐含时间延迟，b波隐含时间显著增加。注射stz的E18胚胎光感受器培养的l型电压门控钙通道（L-VGCC）电流显著降低，这反映在stz -糖尿病视网膜中L-VGCCα 1d亚基水平的降低。通过这些独立的证据线，stz注射液能够诱导鸡胚胎视网膜的病理状态，有望将鸡作为1型糖尿病的潜在新动物模型。
Diabetic retinopathy (DR) is the leading cause of blindness among the American working population. The purpose of this study is to establish a new diabetic animal model using a cone-dominant avian species to address the distorted color vision and altered cone pathway responses in prediabetic and early diabetic patients. Chicken embryos were injected with either streptozotocin (STZ), high concentration of glucose (high-glucose), or vehicle at embryonic day 11. Cataracts occurred in varying degrees in both STZ- and high glucose-induced diabetic chick embryos at E18. Streptozotocin-diabetic chicken embryos had decreased levels of blood insulin, glucose transporter 4 (Glut4), and phosphorylated protein kinase B (pAKT). In STZ-injected E20 embryos, the ERG amplitudes of both a- and b-waves were significantly decreased, the implicit time of the a-wave was delayed, while that of the b-wave was significantly increased. Photoreceptors cultured from STZ-injected E18 embryos had a significant decrease in L-type voltage-gated calcium channel (L-VGCC) currents, which was reflected in the decreased level of L-VGCCα1D subunit in the STZ-diabetic retinas. Through these independent lines of evidence, STZ-injection was able to induce pathological conditions in the chicken embryonic retina, and it is promising to use chickens as a potential new animal model for type I diabetes.
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