Indirect effects of insect herbivory on leaf gas exchange in soybean
Indirect effects of insect herbivory on leaf gas exchange in soybean
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DOI：
10.1111/j.1365-3040.2005.01279.x
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发表时间：
2005-03-01
影响因子：
7.3
通讯作者：
DeLucia, EH
中科院分区：
文献类型：
--
作者：
Aldea, M;Hamilton, JG;DeLucia, EH
食草可以通过去除光合作用的叶子组织来直接影响植物的碳增益，也可以通过诱导昂贵的防御化合物的产生或破坏水和养分的运动来间接影响植物的碳增益。利用气体交换、叶绿素荧光和热成像研究了食草对大豆叶片碳和水通量的间接影响。 Popillia japonica 和 Helicoverpa zea (Boddie) 的食草导致大豆小叶的蒸腾量增加 20-90%，而不影响碳同化率或光合效率 (Phi(PSII))。静脉间组织的机械损伤使蒸腾量增加高达 150%。叶子温度的空间模式表明，水分流失是由于角质层损伤以及切割边缘而发生的。荧光示踪剂（磺基罗丹明 G）表明，水从距离受损叶片切边约 100 微米的质外体中蒸发。受损叶片的失水率在 6 天内仍显着高于对照叶片，在此期间，它们比对照叶片损失的水分多 45%（每厘米受损周长 0.72 mol H2O）。受损组织周围的大量失水表明食草可能会加剧田间条件下大豆的水分胁迫。
Herbivory can affect plant carbon gain directly by removing photosynthetic leaf tissue and indirectly by inducing the production of costly defensive compounds or disrupting the movement of water and nutrients. The indirect effects of herbivory on carbon and water fluxes of soybean leaves were investigated using gas exchange, chlorophyll fluorescence and thermal imaging. Herbivory by Popillia japonica and Helicoverpa zea (Boddie) caused a 20-90% increase in transpiration from soybean leaflets without affecting carbon assimilation rates or photosynthetic efficiency (Phi(PSII)). Mechanical damage to interveinal tissue increased transpiration up to 150%. The spatial pattern of leaf temperature indicated that water loss occurred from injuries to the cuticle as well as from cut edges. A fluorescent tracer (sulforhodamine G) indicated that water evaporated from the apoplast approximately 100 mum away from the cut edges of damaged leaves. The rate of water loss from damaged leaves remained significantly higher than from control leaves for 6 d, during which time they lost 45% more water than control leaves (0.72 mol H2O per cm of damaged perimeter). Profligate water loss through the perimeter of damaged tissue indicates that herbivory may exacerbate water stress of soybeans under field conditions.