Manipulating location, polarity, and outgrowth length of neuron-like pheochromocytoma (PC-12) cells on patterned organic electrode arrays
Manipulating location, polarity, and outgrowth length of neuron-like pheochromocytoma (PC-12) cells on patterned organic electrode arrays
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DOI：
10.1039/c1lc20675c
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Chen, Peilin
中科院分区：
文献类型：
--
作者：
Hsiao, Yu-Sheng;Lin, Chung-Chih;Chen, Peilin
在本文中，我们描述了一种生物相容性有机电极系统，包括在氧化铟锡（ITO）玻璃上的聚（3,4-乙烯二氧噻吩）（PEDOT）微电极阵列，可用于调节神经元类型，位置，极性和神经元样细胞的生长长度（PC-12）。我们通过电化学聚合制备了四种不同尺寸（扁平、20、50和100 μ m）的PEDOT微电极阵列。细胞外基质蛋白在这些有机电极上被很好地吸收；采用聚氧聚乙烯/聚氧聚丙烯/聚氧聚乙烯三嵌段共聚物（PEO/PPO/PEO, Pluronic (TM) F108）作为抗粘涂层时，电池在有机电极上选择性吸附。在该系统中，可以通过改变PEDOT微电极阵列的宽度来控制神经突极性和神经元类型。在无图案PEDOT电极上，PC-12细胞被随机极化，大约80%的细胞具有多极细胞类型。当我们在20 μ m宽的PEDOT线阵列上培养PC-12细胞时，神经突沿有机电极方向排列，单极和双极PC-12细胞的百分比增加到90%以上。施加电刺激后，微电极上的神经突的生长速度提高了60%。因此，这些电活性PEDOT微电极阵列在与哺乳动物神经系统发育和再生相关的组织工程中具有潜在的应用前景。
In this manuscript, we describe a biocompatible organic electrode system, comprising poly(3,4-ethylenedioxythiophene) (PEDOT) microelectrode arrays on indium tin oxide (ITO) glass, that can be used to regulate the neuron type, location, polarity, and outgrown length of neuron-like cells (PC-12). We fabricated a PEDOT microelectrode array with four different sizes (flat; 20, 50, and 100 mu m) through electrochemical polymerization. Extracellular matrix proteins absorbed well on these organic electrodes; cells absorbed selectively on the organic electrodes when we used polyethylene oxide/polypropylene oxide/polyethylene oxide triblock copolymers (PEO/PPO/PEO, Pluronic (TM) F108) as the anti-adhesive coating. In this system, the neurite polarities and neuron types could be manipulated by varying the width of the PEDOT microelectrode arrays. On the unpatterned PEDOT electrode, PC-12 cells were randomly polarized, with approximately 80% having multi-polar cell types. In contrast, when we cultured PC-12 cells on the 20 mu m wide PEDOT line array, the neurites aligned along the direction of the organic electrodes, with the percentage of uni- and bipolar PC-12 cells increasing to greater than 90%. The outgrowth of neurites on the microelectrodes was promoted by similar to 60% with an applied electrical stimulation. Therefore, these electroactive PEDOT microelectrode arrays have potential for use in tissue engineering related to the development and regeneration of mammalian nervous systems.