Cell-mediated immunity to gluten within the small intestinal mucosa in coeliac disease.
Cell-mediated immunity to gluten within the small intestinal mucosa in coeliac disease.
复制标题
乳糜泻小肠粘膜内细胞介导的麸质免疫。
作者：
P. Howdle;A. Bullen;M. Losowsky
来自对照组和乳糜泻患者的空肠活组织检查在存在谷蛋白组分III的情况下保持在器官培养物中。在使用正常外周血白细胞的迁移抑制试验中，测定培养基中淋巴因子活性的证据。与对照组（P小于0.005）或治疗的腹腔患者（P小于0.001）相比，未治疗的腹腔患者的培养基产生显著的迁移抑制，表明未治疗的腹腔粘膜响应于谷蛋白组分III产生白细胞迁移抑制因子（LIF）。抑制程度与腹腔活检组织中培养前上皮下淋巴细胞计数相关（P <0.02）。在6名腹腔患者中，当他们接受正常饮食和无麸质饮食时，LIF在正常饮食时产生，而在无麸质饮食时则不产生。这些结果表明，在未经治疗的乳糜泻患者的粘膜中存在对麸质的局部细胞介导的免疫反应，但这可以通过无麸质饮食治疗逆转。
Jejunal biopsies from controls and coeliac patients were maintained in organ culture in the presence of gluten fraction III. The culture media were assayed for evidence of lymphokine activity in a migration inhibition test using normal peripheral blood leucocytes. Significant inhibition of migration was produced by media from untreated coeliac patients compared with controls (P less than 0.005) or treated coeliac patients (P less than 0.001), indicating the production of a leucocyte migration inhibition factor (LIF) by untreated coeliac mucosa in response to gluten fraction III. The degree of inhibition correlated with the preculture interepithelial lymphocyte count in the coeliac biopsies (P less than 0.02). In six coeliac patients studied when on a normal diet and on a gluten-free diet, LIF was produced while on a normal diet, but not while on a gluten-free diet. These results suggest that a local cell-mediated immune reaction to gluten is present in the mucosa of patients with untreated coeliac disease but that this is reversed by treatment with a gluten-free diet.