Cytotoxicity of a BIS-GMA dental composite before and after leaching in organic solvents.
Cytotoxicity of a BIS-GMA dental composite before and after leaching in organic solvents.
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BIS-GMA 牙科复合材料在有机溶剂中浸出前后的细胞毒性。
DOI：
10.1002/jbm.820250403
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Filisko,FE
中科院分区：
文献类型：
--
作者：
Rathbun,MA;Craig,RG;Hanks,CT;Filisko,FE
细胞培养技术用于确定商业 BIS-GMA 复合材料中细胞毒性剂的来源。该材料根据制造商的说明进行聚合，并通过在乙醇、氯仿或甲苯中进行室温萃取来去除可浸出组分。使用红外光谱分析鉴定提取物中的可浸出成分。使用薄层色谱分析来确定成分的数量。这些成分通过气相色谱法分离，然后通过质谱分析进行鉴定。琥珀酸脱氢酶活性和氚化亮氨酸放射性标记分别用于评估细胞代谢和蛋白质合成。提取物的红外分析表明主要成分是未反应的 BIS-GMA。通过质谱方法检测到痕量的光稳定剂 2-羟基-4-甲氧基-二苯甲酮以及可能从 BIS-GMA 降解的苯甲酸苯酯。与未提取的 BIS-GMA 样品相比，去除可浸出成分后毒性降低了 90%。与 Teflon 阴性对照相比，提取的 BIS-GMA 样品没有显示出细胞反应。
Cell culture techniques were used to determine the source of cytotoxic agents in a commercial BIS‐GMA composite. The material was polymerized according to the manufacturer′s directions and leachable components were removed by room temperature extraction in ethanol, chloroform, or toluene. The leachable components in the extracts were identified using infrared spectrographic analysis. Thin layer chromatographic analysis was used to determine the number of constituents. These constituents were separated by gas chromatography and then identified by mass spectrographic analysis. Succinic dehydrogenase activity and radioactive labeling with tritiated leucine were used to evaluate cell metabolism and protein synthesis, respectively. The infrared analysis of the extracts showed that the primary component was unreacted BIS‐GMA. Trace amounts of 2‐hydroxy‐4‐methoxy‐benzophenone, a light stabilizer, as well as a phenyl ester of benzoic acid which was probably degraded from BIS‐GMA, were detected by the mass spectrographic method. The removal of leachable components caused a 90% decrease in toxicity compared to the nonextracted BIS‐GMA samples. The extracted BIS‐GMA samples showed no cellular response compared to the Teflon negative control.