Effect of different glass ionomers on the acid production and electrolyte metabolism of Streptococcus mutans Ingbritt.

Effect of different glass ionomers on the acid production and electrolyte metabolism of Streptococcus mutans Ingbritt.
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不同玻璃离聚物对变形链球菌 Ingbritt 产酸和电解质代谢的影响。

DOI:
10.1159/000261483
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发表时间:
1992
期刊:
影响因子:
4.2
通讯作者:
H. Luoma
H. Luoma
中科院分区:
医学2区
文献类型:
--
作者:
L. Seppä;E. Torppa;H. Luoma

文献摘要

被引文献

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为了研究不同玻璃离子材料对变形链球菌代谢的影响,将新鲜混合的普通玻璃离子材料(富士)、银玻璃离子材料(Ketac-Silver)、复合玻璃离子材料(Silux)和2周龄的富士玻璃离子材料放入试管底部。将变形链球菌Ingbritt菌株的菌斑样层离心到试验板上,在1.7%(w/v)的蔗糖溶液中孵育20h。对于新鲜混合的富士和酮-银,pH降幅明显小于旧富士和复合。这些材料还将最大数量的氟化物释放到流体中。与玻璃离子材料孵育导致细菌内氟的细胞浓度增加,但细胞内氟与pH下降无关。PH值越低,细胞内镁含量越低。Ketac-Silver在液态中释放大量钙,这组细胞的钙含量增加了一倍。结果表明,新鲜混合玻璃离子在体外对变形链球菌的产酸和电解质代谢有影响。然而,传统玻璃离子的效果似乎在几周后就消失了。金属陶瓷玻璃离子对钙、银释放的影响值得进一步研究。
In order to study the effects of different glass ionomers on the metabolism of Streptococcus mutans, test slabs of freshly mixed conventional glass ionomer (Fuji), silver glass ionomer (Ketac-Silver), composite (Silux), and 2-week-old Fuji were fitted into the bottom of a test tube. A plaque-like layer of S. mutans strain Ingbritt was centrifuged onto the test slabs, and the samples were incubated for 20 h in 1.7% (w/v) sucrose solution. For freshly mixed Fuji and Ketac-Silver, the pH fall was significantly smaller than for old Fuji and composite. These materials also released the largest amount of fluoride into the fluid phase. Incubation with glass ionomer materials led to an increase in the cellular concentration of fluoride in bacteria, but intracellular fluoride did not correlate with the fall in pH. The lowest pH was associated with the lowest cellular magnesium content. Ketac-Silver released large amounts of calcium in the fluid phase, and the cellular calcium content was doubled in this group. The results show that freshly mixed glass ionomers affect acid production and electrolyte metabolism of S. mutans in vitro. The effect of conventional glass ionomer, however, seems to disappear after a few weeks. The effects of calcium and silver released by cermet glass ionomer deserve further study.