Endometriotic cyst fluid induces reactive oxygen species (ROS) in human immortalized epithelial cells derived from ovarian endometrioma
Endometriotic cyst fluid induces reactive oxygen species (ROS) in human immortalized epithelial cells derived from ovarian endometrioma
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DOI：
10.1080/13510002.2016.1258448
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发表时间：
2017-01-01
期刊：
影响因子：
3.8
通讯作者：
Iwashita, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Kobayashi, Yoichi;Osanai, Kiyono;Iwashita, Mitsutoshi
目的：子宫内膜异位囊肿液（ECF）中含有大量活性氧（ROS），子宫内膜异位囊肿暴露于强氧化应激中，可引起恶变。方法：用ECF处理来源于卵巢上皮瘤的人永生化上皮细胞（EMosis-CC/TERT 1），观察ECF对细胞内ROS产生的影响。结果：共获得38例诊断为乳腺增生性囊肿的ECF标本。在EMosis-CC/TERT 1中，ECF诱导的ROS水平显著高于溶剂对照和次氮基三乙酸铁。ROS产生的偏侧性和术前血清CA 125值没有显着差异。有几个患者的囊肿大小约为5cm，并具有相对较高的ROS产生。ECF产生的ROS在年龄大于40岁的患者中相对高于年龄小于40岁的患者。讨论：我们的研究表明ECF在EMosis-CC/TERT 1细胞中产生大量ROS，因此，暴露于ECF可诱导强烈的氧化应激。开发一种治疗策略，以减少ROS的产生可能是有用的，以防止恶性转化的囊肿。
Objectives: Endometriotic cyst fluid (ECF) contains a large amount of reactive oxygen species (ROS), and endometriotic cysts are exposed to strong oxidative stress, which may cause malignant transformation. In this study, ROS production by ECF was clinically analysed.Methods: Human immortalized epithelial cells derived from ovarian endometrioma (EMosis-CC/TERT 1) were treated with ECF. In addition, ROS production in EMosis-CC/TERT 1 was measured, and its clinical significance was analysed.Results: A total of 38 ECF samples were obtained from patients diagnosed with endometriotic cysts. In EMosis-CC/TERT1, significantly higher levels of ROS were induced by ECF than by the vehicle control and ferric nitrilotriacetate. There were no significant differences in ROS production by laterality and preoperative serum CA125 values. There were several patients whose cyst sizes were approximately 5cm and had relatively high ROS production. Production of ROS by ECF was relatively higher in patients older than 40 years of age than in those younger than 40.Discussion: Our study revealed that ROS are highly produced by ECF in EMosis-CC/TERT1 cells; therefore, exposure to ECF induced strong oxidative stress. Development of a therapeutic strategy to reduce ROS production might be useful for preventing malignant transformation of endometriotic cysts.