Patterning of virus-infected Glycine max seed coat is associated with suppression of endogenous silencing of chalcone synthase genes
Patterning of virus-infected Glycine max seed coat is associated with suppression of endogenous silencing of chalcone synthase genes
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DOI：
10.1105/tpc.019885
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发表时间：
2004-04-01
期刊：
影响因子：
11.6
通讯作者：
MacFarlane, S
中科院分区：
文献类型：
--
作者：
Senda, M;Masuta, C;MacFarlane, S
大多数商业大豆（大豆）品种由于种皮中色素的损失而具有黄色种子。研究表明，抑制黄曲霉种皮色素沉着是一种有效的方法。max的表达可能受查耳酮合酶（CHS）基因的同源依赖性沉默控制。我们对CHS mRNA和短干扰RNA的分析提供了明确的证据，即抑制黄G. max的结果从转录后，而不是转录沉默的CHS基因。此外，我们发现，斑驳症状存在于种皮G。被某些病毒感染的max植物可通过病毒沉默抑制蛋白抑制CHS转录后基因沉默（PTGS）而引起。这些结果表明，天然存在的PTGS在植物中的独特表型的表达中起着关键作用，并提出了一个简单明确的例子，阐明了病毒症状诱导的分子机制。
Most commercial Glycine max (soybean) varieties have yellow seeds because of loss of pigmentation in the seed coat. It has been suggested that inhibition of seed coat pigmentation in yellow G. max may be controlled by homology-dependent silencing of chalcone synthase (CHS) genes. Our analysis of CHS mRNA and short-interfering RNAs provide clear evidence that the inhibition of seed coat pigmentation in yellow G. max results from posttranscriptional rather than transcriptional silencing of the CHS genes. Furthermore, we show that mottling symptoms present on the seed coat of G. max plants infected with some viruses can be caused by suppression of CHS posttranscriptional gene silencing (PTGS) by a viral silencing suppressor protein. These results demonstrate that naturally occurring PTGS plays a key role in expression of a distinctive phenotype in plants and present a simple clear example of the elucidation of the molecular mechanism for viral symptom induction.