Promotion of the rapid growth in Haematococcus pluvialis under 0.16% CO2 condition revealed by transcriptome and metabolomic analysis
Promotion of the rapid growth in Haematococcus pluvialis under 0.16% CO2 condition revealed by transcriptome and metabolomic analysis
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促进%20of%20the%20rapid%20growth%20in%20红球菌%20pluvialis%20under%200.16%%20CO2%20condition%20revealed%20by%20transcriptome%20and%20metabolomic%20analysis
DOI：
10.1007/s00344-019-10055-6
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发表时间：
2019
影响因子：
4.8
通讯作者：
Xue Sun
中科院分区：
文献类型：
--
作者：
Xin Ye;Jiani Chen;Chaoyang Hu;Nianjun Xu;Xue Sun
单细胞绿藻雨红球菌被认为是虾青素的最佳天然来源，虾青素是一种强抗氧化剂。在本研究中，h。在co2浓度为0.16%和0.04%的条件下进行了淋生实验，以探讨co2对该藻类营养阶段生长的影响机制。通过转录组分析，共筛选出1665个不同co2条件下的差异表达基因。与光合作用、三羧酸（TCA）循环、糖酵解、戊糖磷酸途径和氮代谢相关的基因在0.16% CO2环境下大部分上调。代谢谱共鉴定出36种差异代谢物，其中柠檬酸和核糖积累较多；然而，在0.16%的co2水平下，12种常见氨基酸和鸟氨酸、腐胺等抗逆性相关底物减少。结合藻类生长的结果，co2的升高促进了光合作用和碳利用，包括TCA循环和糖酵解，同时刺激了氮代谢、蛋白质合成和能量代谢，导致h的快速生长。pluvialis。
The unicellular green algaHaematococcus pluvialisis considered the optimal natural source of astaxanthin, a strong antioxidant in nature. In the present study, transcriptome and metabolic profiling ofH. pluvialisunder 0.16% and 0.04% CO2levels were performed to explore the underlying mechanism by which CO2affects growth at the vegetative stage of this alga. Approximately 1665 differentially expressed unigenes were screened in response to different CO2conditions by transcriptome analysis. The genes related to photosynthesis, the tricarboxylic acid (TCA) cycle, glycolysis, pentose phosphate pathway, and nitrogen metabolism, were mostly up-regulated by 0.16% CO2. A total of 36 differential metabolites were identified in metabolic profiling, of them, citric acid and ribose were accumulated; however, 12 common amino acids and stress-resistant related substrates such as ornithine and putrescine were decreased at 0.16% CO2level. Combing the results of the algal growth, the elevated CO2promoted photosynthesis, and carbon utilization including TCA cycle and glycolysis, together with the stimulated nitrogen metabolism, protein synthesis, and energy metabolism, which resulted in rapid growth ofH. pluvialis.