Oenococcus kitaharae sp nov., a non-acidophilic and non-malolactic-fermenting oenococcus isolated from a composting distilled shochu residue
Oenococcus kitaharae sp nov., a non-acidophilic and non-malolactic-fermenting oenococcus isolated from a composting distilled shochu residue
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DOI：
10.1099/ijs.0.64288-0
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发表时间：
2006-10-01
影响因子：
2.8
通讯作者：
Okada, Sanae
中科院分区：
文献类型：
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作者：
Endo, Akihito;Okada, Sanae
从日本烧酒残渣堆肥中分离出6株乳酸菌。6株菌株在MRS琼脂上生长不良，在MRS肉汤中生长缓慢。16S rRNA基因序列与已知乳酸菌种的相似性不高，在与酒酒球菌密切相关的专性异发酵乳酸菌群中形成了一个亚簇。DNA-DNA亲缘关系水平表明，分离物属于同一分类单元，在遗传上与O. oeni分离。此外，各种表型特征，如生长的最佳pH、苹果酸乳酸发酵和对10%乙醇的抗性，表明分离物与O. oeni有区别。根据它们的系统发育和表型特征，这些分离物代表了一个新种，建议将其命名为Oenococcus kitaharae sp. 11。型应变为NRIC 0645 T (=JCM 13282（T) =DSM 17330(T)）。
Six strains of lactic acid bacteria were isolated in Japan from a composting distilled shochu residue. The six isolates grew poorly on MRS agar and slowly in MRS broth. The 16S rRNA gene sequences did not show high levels of similarity to those of the recognized species of lactic acid bacteria, and formed a subcluster within the cluster comprising obligately heterofermentative lactic acid bacteria closely related to Oenococcus oeni. The levels of DNA-DNA relatedness revealed that the isolates belonged to the same taxon and were genetically separate from O. oeni. Furthermore, various phenotypic characteristics such as the optimum pH for growth, malolactic fermentation and resistance to 10% ethanol revealed that the isolates are distinguishable from O. oeni. On the basis of their phylogenetic and phenotypic characteristics, the isolates represent a novel species, for which the name Oenococcus kitaharae sp. nov. is proposed. The type strain is NRIC 0645 T (=JCM 13282(T) =DSM 17330(T)).