Peroxynitrite induces apoptosis and decline in intracellular free Mg with concomitant elevation in [Ca2+]I in rat aortic smooth muscle cells: possible roles of extracellular and intracellular magnesium ions in peroxynitrite-induced cell death.
Peroxynitrite induces apoptosis and decline in intracellular free Mg with concomitant elevation in [Ca2+]I in rat aortic smooth muscle cells: possible roles of extracellular and intracellular magnesium ions in peroxynitrite-induced cell death.
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DOI：
10.2174/187231207780363651
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发表时间：
2007-03
影响因子：
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通讯作者：
Jianfeng Li;Wenyan Li;Weimin Liu;B. T. Altura;B. Altura
中科院分区：
文献类型：
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作者：
Jianfeng Li;Wenyan Li;Weimin Liu;B. T. Altura;B. Altura
目前的研究表明，外源性ONOO（-）可导致细胞内游离镁离子（[Mg（2+）]（i））迅速下降，同时细胞内游离钙离子（[Ca（2+）]（i））迅速上升，随后引发大鼠主动脉平滑肌细胞凋亡，但不引起坏死;高[Mg（2+）]显著减弱ONOO（-）诱导的细胞凋亡。ONOO（-）诱导的血管平滑肌细胞凋亡似乎涉及Ca（2+）-Mg（2+）依赖性核酸内切酶和caspase-3的激活。缺镁本身不能诱导SMCs凋亡，但能显著增强ONOO（-）诱导的SMCs凋亡。
The present study demonstrates that exogenous ONOO(-) can result in rapid declines in intracellular free magnesium ions ([Mg(2+)](i)) concomitant with rapid rises in intracellular free calcium ions ([Ca(2+)](i)) and, subsequently, trigger apoptosis but not necrosis in rat aortic SMCs; high [Mg(2+)] significantly attenuates ONOO(-)-induced apoptosis. ONOO(-)-induced apoptosis in vascular SMCs appears to involve activation of Ca(2+)-Mg(2+)-dependent endonucleases and caspase-3. Mg deficiency itself could not induce apoptosis in these SMCs, but it could significantly enhance ONOO(-)-induced apoptosis.